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E0771 WXS

ABSTRACT: E0771 whole exom sequencing

PROVIDER: PRJEB30681 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	ERR3048430.fastq.gz	Fastqsanger.gz
	ERR3048427.fastq.gz	Fastqsanger.gz
	ERR3048428.fastq.gz	Fastqsanger.gz
	ERR3048429.fastq.gz	Fastqsanger.gz

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Gene expression profile at single cell level of CD45+ immune cells from E0771-Vector control tumor tissue, E0771 wildtype Malt1 overexpression tumor tissue and E0771 paracaspase-deficient Malt1 overexpression tumor tissue.

Project description:To invesitigate the additional paracaspase-independent tumor-promoting effect exist for Malt1. We thus collected E0771-Vector control tumor tissue, E0771 wildtype Malt1 (Malt1-WT) overexpression tumor tissue and E0771 paracaspase-deficient Malt1 (Malt1-PD) overexpression tumor tissue, and sorted out immune cells for 10× Genomics single cell RNA-sequencing (scRNA-seq).

2024-04-18 | GSE242554 | GEO

RNA-seq of E0771-Vector control, E0771-Malt1-WT and E0771 Malt1-PD after T cell killing

Project description:RNA-seq of E0771-Vector control, E0771-Malt1-WT and E0771 Malt1-PD after T cell killing

| PRJNA1014469 | ENA

RNA-seq of E0771-Vector control, E0771-Malt1-WT and E0771 Malt1-PD (Without T cell coculture)

Project description:RNA-seq of E0771-Vector control, E0771-Malt1-WT and E0771 Malt1-PD (Without T cell coculture)

| PRJNA1015214 | ENA

RNA-seq of E0771-Vector control and E0771-Malt1-WT at basal condition

Project description:To reveal the downstream signal change induced by Malt1, we collected mRNA samples from Vector control- and WT-Malt1-expressing E0771 cancer cells, and performed RNA-seq analysis.

2024-04-26 | GSE265893 | GEO

Transcriptomic analysis of E0771 tumors treated with FEC + oHSV-1 Vs PBS

Project description:E0771 tumors were implanted either subcutaneously on the left flank or orthotopically in the mammary fat pad and treated with either PBS or FEC + oHSV-1 Mice were sacrificed 5 days after the start of treatment and whole tumour digests were used for RNA extraction

2021-10-01 | GSE183864 | GEO

RNA-seq of E0771-Vector control, E0771-Malt1-WT and E0771 Malt1-PD (Without T cell coculture)

Project description:To reveal the downstream paracrine signals from WT-Matl1 or PD-Malt1 tumor cells which responsible for the above observed macrophage phenotypes, we collected mRNA samples from Vector control-, WT-Malt1-, PD-Malt1-, V87R-Malt1-, or L88D-Malt1-expressing E0771 cancer cells, and performed RNA-seq analysis.

2024-04-18 | GSE242878 | GEO

Intracellular zinc protects tumors from T cell-mediated cytotoxicity [E0771 RNAseq]

Project description:We performed RNA sequencing on parental (WT) and Cbfb-deficient (CbfbKO) E0771 cells. Cells were generated by electroporation with Cas9-sgRNA complexes using a non-targeting sgRNA (WT) or Cbfb-targeting sgRNA (CbfbKO)

2024-10-09 | GSE244138 | GEO

Project description:Whole exom sequencing

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RNA-seq of E0771-Vector control, E0771-Malt1-WT and E0771 Malt1-PD after T cell killing

Project description:To understand potential downstream signaling molecules that are responsible for wild type-Malt1 induced resistance of tumor cell to CD8 T cell killing , we first use E0771 cells expressing either -Vector control, wild type-Malt1 (M-WT), or Malt1 PD mutant (M-PD) to coculture with activated CD8 T cell at a effctor: target ratio(E:T ration) of 3 for 12 hours. Then wash the cells with PBS, digest the cells by 0.25% trypsin solution, harvest the cells in 15 ml tubes by centrifugation (1000 rpm 5min) and seed cells into the original plate with DMEM+10%FBS+1%P/S medium. 6 hours after sseding, wash cells with PBS, lyse cells with TRIZOL for RNA extraction, perform reverse transcription and cDNA library for RNA-Seq analysis.

2024-04-18 | GSE242764 | GEO

Transcriptional changes induced by Lamin A/C downregulation in the nuclei of E0771 breast cancer cells

Project description:Major transcriptional changes (290 differentially expressed genes) take place upon downregulation of Lamin A/C in E0771 breast cancer cells

2021-06-02 | GSE151105 | GEO

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