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RNA-seq of E0771-Vector control and E0771-Malt1-WT at basal condition

ABSTRACT: To reveal the downstream signal change induced by Malt1, we collected mRNA samples from Vector control- and WT-Malt1-expressing E0771 cancer cells, and performed RNA-seq analysis.

ORGANISM(S): Mus musculus

PROVIDER: GSE265893 | GEO | 2024/04/26

REPOSITORIES: GEO

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RNA-seq of E0771-Vector control, E0771-Malt1-WT and E0771 Malt1-PD (Without T cell coculture)

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Gene expression profile at single cell level of CD45+ immune cells from E0771-Vector control tumor tissue, E0771 wildtype Malt1 overexpression tumor tissue and E0771 paracaspase-deficient Malt1 overexpression tumor tissue.

Project description:To invesitigate the additional paracaspase-independent tumor-promoting effect exist for Malt1. We thus collected E0771-Vector control tumor tissue, E0771 wildtype Malt1 (Malt1-WT) overexpression tumor tissue and E0771 paracaspase-deficient Malt1 (Malt1-PD) overexpression tumor tissue, and sorted out immune cells for 10× Genomics single cell RNA-sequencing (scRNA-seq).

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Project description:RNA-seq of E0771-Vector control, E0771-Malt1-WT and E0771 Malt1-PD after T cell killing

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RNA-seq of E0771-Vector control, E0771-Malt1-WT and E0771 Malt1-PD (Without T cell coculture)

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Project description:In lymphocyte lineages, mucosa-associated lymphoid tissue 1 (MALT1) mediates the nuclear factor-M-NM-:B activation signal that stimulates progression of malignant tumors. However, its expression is inactivated in oral carcinoma patients with worse prognosis. Unveiling genes under the control of MALT1 will provide valuable information for understanding of the mechanism of carcinoma progression. We profiled gene datasets altered in response to MALT1 in oral carcinoma cells by the microarray analysis. Totally, 2,933 genes with a fold change M-bM-^IM-% M-BM-12.0 and P < 0.05 were identified. HSC2 oral carcinoma cells were used for RNA extraction and hybridization on Affimetrix microarrays. We sought to obtain homogenous populations of MALT1-resonsive genes in order to increase the resolution of expression profiles. To this end, we used HSC2 cells stably expressing MALT1 (n=3) and vector alone (n=3) for the analysis.

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Expression data for MALT1-responsive genes

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Project description:E0771 whole exom sequencing

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