Project description:Local breeds retained unique genetic variability important for adaptive potential especially in light of challenges related to climate change. Our objective was to perform, for the first time, a genome-wide diversity characterization using Illumina GoatSNP50 BeadChip of autochthonous Drežnica goat breed from Slovenia. Genetic diversity analyses revealed that the Slovenian Drežnica goat has a distinct genetic identity and is closely related to the neighboring Austrian and Italian alpine breeds. These results expand our knowledge on phylogeny of goat breeds from easternmost part of the European Alps.
Project description:The aim of the study was to investigate differences in the gene expression profiles of selected tissues in two most popular goat’s breeds in Poland: Polish White Improved (PWI) and Polish Fawn Improved (PFI). Three different types of tissue samples were selected: somatic cells isolated from goats’ milk (MSC), milk fat globules (MFG) and peripheral nuclear blood cells (PBNC) Since there were no earlier genetic studies focused on genetic differences between these two goat breeds we decided to evaluate hypothetical genomic differences assuming that such a differences should be the consequence of genetic differences. We created the hypothesis that if genomic differences exist they should be revealed in hierarchical clustering of transcriptomic profiles of selected tissues. Should the genomic differences exist the clusters obtained are grouping goat breeds and not goat’s tissues. The results of hierarchical clustering however show something completely different. The clusters are grouping goat tissues (milk fat globules, milk somatic cells, peripheral blood nuclear cells) without any relation with goat breed. So the analytical tool does not recognize the goat breed as a driver of transcriptomic difference. Moreover, we were not able to find significantly regulated genes between two breeds
Project description:Goats are adapted efficiently in different agro-climatic conditions. Goat milk has several unexplored health-promoting properties that need to be investigated scientifically and promoted commercially. Goat milk is mainly consumed in family and plays a pivotal role in fulfilling the nutritional requirement of older people, pregnant women and children. There has been considerable interest in goat milk protein due to availability of different bioactive peptides, which have nutraceutical applications. Secondly, goat milk is being used for treatment of different disease, allergy and heat stress condition. Therefore, the milk proteome analysis appears to be timely and required for future industrial application with respect to human health and nutrition. 2-DGE analysis of identified protein variants was performed and further identification of proteins was carried out through LC-MS/MS. variant samples from 15 breeds were resolved by 2DGE and spots were identified and analysed by LC-MS/MS from 15 different breeds for presence of unique peptides.
Project description:The present study, for the first time, compared the transcriptomes of ovaries from the prolific Jintang black goat and the non-prolific Tibetan goat during follicular phase using the Illumina RNA-Seq method. The study provides insight into the transcriptional regulation in the ovaries of two distinct breeds of goats that might serve as a key resource for understanding goat fecundity.
Project description:Assessing the productive and adaptive capability of two different goat breeds to heat stress based on differences in the phenotypic and genotypic traits
| PRJNA795896 | ENA
Project description:Assessing the productive and adaptive capability of two different goat breeds to heat stress based on differences in the phenotypic and genotypic traits