Ontology highlight
ABSTRACT:
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| ERR202898_1.fastq.gz | Fastqsanger.gz | |||
| ERR202898_2.fastq.gz | Fastqsanger.gz | |||
| ERR202899_1.fastq.gz | Fastqsanger.gz | |||
| ERR202899_2.fastq.gz | Fastqsanger.gz | |||
| ERR202900_1.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 30 |
Similar Datasets
Project description:Eusocial insects have evolved the capacity to generate adults with distinct morphological, reproductive and behavioural phenotypes from the same genome. Recent studies suggest that RNA editing might enhance the diversity of gene products at the post-transcriptional level, particularly to induce functional changes in the nervous system. Using head samples from the leaf-cutting ant Acromyrmex echinatior, we compare RNA editomes across eusocial castes, identifying ca. 11,000 RNA editing sites in gynes, large workers and small workers. Those editing sites map to 800 genes functionally enriched for neurotransmission, circadian rhythm, temperature response, RNA splicing and carboxylic acid biosynthesis. Most A. echinatior editing sites are species specific, but 8M-bM-^@M-^S23% are conserved across ant subfamilies and likely to have been important for the evolution of eusociality in ants. The level of editing varies for the same site between castes, suggesting that RNA editing might be a general mechanism that shapes caste behaviour in ants. Analysis of genome-wide RNA editing in three different female castes of the the leaf-cutting ant Acromyrmex echinatior.
2014-10-02 | E-GEOD-51576 | biostudies-arrayexpress
Project description:Integument of leaf-cutting ants Raw sequence reads
| PRJNA321130 | ENA
Project description:Leaf-cutting ants of the genera Acromyrmex and Atta live in mutualistic symbiosis with a basidiomycete fungus (Leucocoprinus gongylophorus), which they cultivate as fungal gardens in underground nest chambers. The ants provide the fungus with a growth substrate consisting of freshly cut leaf fragments. After new leaf fragments are brought into the nest, the ants chew them into smaller pieces and apply droplets of fecal fluid to the leaf pulp before depositing this mixed substrate in the fungus garden and inoculating it with small tufts of mycelium from older parts of the garden. Previous work has shown that the fecal fluid contains a range of digestive enzymes including proteases, amylases, chitinases, cellulases, pectinases, hemicellulases and laccases, and that most of these enzymes are produced by the fungal symbiont in specialized structures called gongylidia that the ants eat. After ingestion, the enzymes apparently pass unharmed through the alimentary channel of the ants and end up in the fecal fluid. Most likely this complex system is an adaptation of the ant-fungus symbiosis to a herbivorous lifestyle, as the ancient ancestors of the ants and the fungus lived as hunter-gatherers and saprotrophs, respectively. The promise of fecal fluid for getting insight into the molecular adaptations that enables the ant-fungus holosymbiont to live as a herbivore, led us to investigate the fecal fluid proteome using LC-MS/MS in order to get a more comprehensive picture of the repertoire of proteins present.
2021-01-12 | PXD016395 | Pride
Project description:Eusocial insects have evolved the capacity to generate adults with distinct morphological, reproductive and behavioural phenotypes from the same genome. Recent studies suggest that RNA editing might enhance the diversity of gene products at the post-transcriptional level, particularly to induce functional changes in the nervous system. Using head samples from the leaf-cutting ant Acromyrmex echinatior, we compare RNA editomes across eusocial castes, identifying ca. 11,000 RNA editing sites in gynes, large workers and small workers. Those editing sites map to 800 genes functionally enriched for neurotransmission, circadian rhythm, temperature response, RNA splicing and carboxylic acid biosynthesis. Most A. echinatior editing sites are species specific, but 8–23% are conserved across ant subfamilies and likely to have been important for the evolution of eusociality in ants. The level of editing varies for the same site between castes, suggesting that RNA editing might be a general mechanism that shapes caste behaviour in ants.
2014-10-02 | GSE51576 | GEO
Project description:Fungus-growing ants microbial interaction of Streptomyces sp. and Escovopsis sp. through molecular networking and MALDI imaging
2018-11-03 | MSV000083097 | MassIVE
Project description:Exopolysaccharide galactosaminogalactan (GAG) is a fungal cell wall component composed of α-1,4 linked galactose, N-acetyl galactosamine and galactosamine, which has been demonstrated in Aspergillus fumigatus in association with fungal adhesion, biofilm formation and virulence. The gene cluster responsible for GAG biosynthesis has only been characterized in Aspergillus fungi. We found that the highly conserved gene cluster for GAG biosynthesis is also present in the insect pathogenic fungi Metarhizium species. Functional investigations in M. robertsii revealed that GAG is only produced on fungal cell wall during fungal germination, filamentation and the formation of the infection structure appressoria. Gene deletions revealed that, relative to the wild-type (WT), the appressorial mucilage production was abolished in the null mutant of M. robertsii. Since multiple enzymes are produced in appressorial mucilages, appressorial samples of the WT and mutant formed on cicada wings were collected and subjected to iTRAQ comparative proteomic analysis. We found that different protein families were up- or down-regulated in the null mutant when compared with the WT.
2021-06-07 | PXD019301 | Pride
Project description:Using TMT-labeled quantitative protein technique, we quantitatively analyzed the proteomes of three different resistant Bambusa pervariabilis × Dendrocalamopisis grandis under four different treatments: pathogenic fungi, pathogenic toxin, pathogenic fungi plus toxin and sterile water, and screened out the differential proteins in different groups.
2019-06-24 | PXD014363 |
Project description:Although most known mycoviruses are asymptomatic or reduce the virulence of their host fungi, those that confer hypervirulence to entomopathogenic fungus still need to be explored. Here, we discovered and studied a novel mycovirus in Metarhizium flavoviride, isolated from Laodelphax striatellus. Based on molecular analysis, we tentatively designated the mycovirus as Metarhizium flavoviride partitivirus 1 (MfPV1), a novel species in genus Gammapartitivirus, family Partitiviridae. MfPV1 has two double-stranded (ds) RNAs as its genome, 1,775 and 1,575 bp in size respectively, encapsidated in isometric particles. When we transfected commercial strains of M. anisopliae and M. pingshaense with MfPV1, conidiation was significantly enhanced (t-test; P-value < 0. 01), and the significantly higher mortality rates of the larvae of Plutella xylostella and Spodoptera frugiperda, two important lepidopteran pests were found in virus-transfected strains (ANOVA; P-value < 0.05). Transcriptomic analysis showed that transcript levels of pathogenesis-related genes in MfPV1-infected M. anisopliae were obviously altered, suggesting increased production of metarhizium adhesin-like protein, hydrolyzed protein and destruxin synthetase. Further studies are required to elucidate the mechanism whereby MfPV1 enhances the expression of pathogenesis-related genes and virulence of Metarhizium to lepidopteran pests. This study presents experimental evidence that the transfection of other entomopathogenic fungal species with a mycovirus can confer significant hypervirulence and provides a good example that mycoviruses could be used as synergistic agent to enhance the biocontrol activity of entomopathogenic fungi.
2024-05-03 | GSE266301 | GEO
Project description:Leaf-cutter ants use fresh plant material to cultivate an obligate mutualistic fungus Leucoagaricus gongylophorus in specialized fungus gardens to access and transform nutrients from plant biomass that would otherwise be unavailable to the ants. Here, we evaluated the lipidomic differences between the leaves feeding the gardens, gongylidia produced by the fungus to feed the ants, and spatially-resolve regions of the fungus garden at initial to advanced stages of leaf degradation.
2019-10-30 | MSV000084520 | MassIVE