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RNAseq from endosperm and pericarp of 3 wheat genotypes: Cadenza, triple stack of TaiRX9b knock-out alleles, null segregant control

ABSTRACT: RNAseq from endosperm and pericarp of 3 wheat genotypes: Cadenza, triple stack of TaiRX9b knock-out alleles, null segregant control

PROVIDER: PRJEB33954 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR3476080_1.fastq.gz	Fastqsanger.gz
	ERR3476080_2.fastq.gz	Fastqsanger.gz
	ERR3476090_1.fastq.gz	Fastqsanger.gz
	ERR3476090_2.fastq.gz	Fastqsanger.gz
	ERR3476100_1.fastq.gz	Fastqsanger.gz

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RNAseq from endosperm and pericarp of 3 wheat genotypes: Cadenza, triple stack of TaiRX9b knock-out alleles, null segregant control

Project description:Using mutagenised population of wheat cv Cadenza, we identified knock-out alleles fro the 3 homeologues of TaIRX9b and stacked them in one line. To look for effects of loss of TaiRX9b gene function on transcriptome we sequenced RNA from endosperm and pericarp from triple stack, null egregant control and unmutagenised Cadenza.

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Transcription profiling of wheat B1118-8 4(6) line with its background control-Cadenza bread wheat line. Transcriptomes analysis was performed in endosperm tissue (14 and 28 days post anthesis-dpa) and in leaf tissue (8 days post germination dpg)

Project description:B1118-8-4 expresses five HMW subunits encoded by Glu-B1 (14, 15) and Glu D1 (5, 10) and transgene Glu-A1 (Ax1).Cadenza does not express Glu-A1 (Ax1. B111-8-8-4 (6)transgenic wheat line was produced by co-transformation with two plasmids: one of the plasmids carring the transgene HMW-GS 1Ax1 (Halford, N.G. et al. Analysis of HMW glutenin subunits encoded bychromosome 1A of bread wheat (Triticum aestivum L.) and the other plasmid harbour the marker genes bar and gus indicates quantitative effects on grain quality. Theor Appl Genet 83, 373-378 (1992).) and the other the bar gus gene sequences. We compared the transcriptome of transgenic B1118-8 4(6) wheat line with its background control-Cadenza bread wheat line. Transcriptomes analysis was performed in endosperm tissue (14 and 28 days post anthesis-dpa) and in leaf tissue (8 days post germination dpg). Each of the transcriptome comparisons was performed using three biological replicates (i.e. per line/tissue/developmental stage selected). Hybridisations were performed in reverse dye labelling.

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Project description:RNAseq from endosperm and pericarp of 3 wheat genotypes: Cadenza, triple stack of TaiRX9b knock-out alleles, null segregant control

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