RNA-seq of liver from Ldlr-/- mouse fed with inulin or cellulose supplemented atherogenic diet
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB34064 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| ERR3486082.fastq.gz | Fastqsanger.gz | |||
| ERR3486083.fastq.gz | Fastqsanger.gz | |||
| ERR3486084.fastq.gz | Fastqsanger.gz | |||
| ERR3486085.fastq.gz | Fastqsanger.gz | |||
| ERR3486086.fastq.gz | Fastqsanger.gz |
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Project description:Dietary fiber such as inulin have been reported to promote cardiovascular and metabolic health. However, the mechanisms involved are not well understood. We studied effects of inulin on lipid metabolism in Ldlr deficient atherosclerosis mouse model using lipidomics and transcriptomics. Plasma and tissues were collected at 10 days and/or 12 weeks after feeding an atherogenic diet supplemented with inulin or cellulose (control).
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Project description:OPP (1500 ppm gallic acid equivalent (GAE)) was supplemented to BALB/c mice given an atherogenic diet for six weeks to observe for possible anti-atherogenic effects. The control group received distilled water instead of OPP. Livers, spleens and hearts were harvested six weeks after the feeding regimen for gene expression studies. Results from the separate microarray data analysis carried out on the different organs show that OPP attenuated the effects of the atherogenic diet in the organs. Total RNA obtained from livers, spleens and hearts of BALB/c mice given OPP (six weeks after administration of an atherogenic diet) were compared to controls given distilled water (liver: three replicates in the treatment group versus four replicates in the control group; spleen and heart: three replicates in the treatment group versus three replicates in the control group for both organs)
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