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Dental calculus of ancient baboons

ABSTRACT: Metagenomic analysis of dental calculus of ancient baboons

PROVIDER: PRJEB34875 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR3609660_1.fastq.gz	Fastqsanger.gz
	ERR3609660_2.fastq.gz	Fastqsanger.gz
	ERR3609661_1.fastq.gz	Fastqsanger.gz
	ERR3609661_2.fastq.gz	Fastqsanger.gz
	ERR3609652_1.fastq.gz	Fastqsanger.gz

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Project description:This study investigated the consumption of milk products in the archaeological record, utilizing human dental calculus as a reservoir of dietary proteins from archaeological samples from across Eurasia. Protein extraction and generation of tryptic peptides from dental calculus was performed using a filter-aided sample preparation (FASP) protocol, modified for ancient samples, on 92 samples of archaeological dental calculus. Samples were extracted at three laboratories; the Functional Genomics Centre Zürich (FGCZ), the Centre for GeoGenetics at the National History Museum of Denmark, and BioArCh at the University of York. Sample extracts were sequenced (LC-MS/MS) using an LTQ-Orbitrap Velos (FGCZ), a Q-Exactive Hybrid Quadrupole Orbitrap and an LTQ-Orbitrap Elite (Central Proteomics Facility, Target Discovery Institute, Oxford).

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