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Insertion profile of IFP2 transposons by the PB transposase

ABSTRACT: Definition using LAM-PCR of the insertion profile of IFP2 transposons containing a NeoR using the wild type PB transposase.

PROVIDER: PRJEB36226 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR3814118_1.fastq.gz	Fastqsanger.gz
	ERR3814118_2.fastq.gz	Fastqsanger.gz

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Insertion profile of IFP2 transposons by a CRD-less PB transposase (1-558)

Project description:Definition using LAM-PCR of the insertion profile of IFP2 transposons containing a NeoR using the wild type CRD-less PB transposase (1-558)

| PRJEB36227 | ENA

Insertion profile of IFP2 transposons by a CRD-less PB transposase (1-558)

Project description:Definition using LAM-PCR of the insertion profile of IFP2 transposons containing a NeoR using the wild type CRD-less PB transposase (1-558) N-terminally fused with a SV40 NLS

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IFP2 transposon by PGBD5 transposase

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Project description:The effect of overexpression of the At3g42170 hAT-like transposase on plant gene expression

2005-03-10 | E-MEXP-279 | biostudies-arrayexpress

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Project description:Deep sequencing of single cell-derived genomic DNA and/or cDNAs brings novel insights into oncogenesis and embryogenesis. However, traditional library preparation for RNA-Seq requires multiple steps, including shearing the target DNA/RNA and following sequential enzymatic reactions, which result in consequent sample loss and stochastic variation at each step. Such variation may significantly affect the output from sequencing. We have found that a new technique of library preparation using hyperactive Tn5 transposase for the next-generation sequencer of Illumina's platform provided high-quality libraries from 100ng of short-length (average 700~800 bp) single-cell level cDNA. This new method reduced the number of steps in the protocol, which resulted in improved reproducibility and reduced variation among the specimens. Two methods of library preparation (sonication, tagmentation with hyperactive Tn5 transposase) were compared in the case of RNA-Seq for single-cell level cDNA. Technical triplicates were used.

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| S-EPMC10983969 | biostudies-literature

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