Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap Velos
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Epithelial Cell
SUBMITTER: Alison Hickman
LAB HEAD: Fred Dyda
PROVIDER: PXD037132 | Pride | 2023-03-11
REPOSITORIES: Pride
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Luo Wentian W Hickman Alison B AB Genzor Pavol P Ghirlando Rodolfo R Furman Christopher M CM Menshikh Anna A Haase Astrid A Dyda Fred F Wilson Matthew H MH
Nucleic acids research 20221201 22
DNA transposon systems are widely used in mammalian cells for genetic modification experiments, but their regulation remains poorly understood. We used biochemical and cell-based assays together with AlphaFold modeling and rational protein redesign to evaluate aspects of piggyBac transposition including the previously unexplained role of the transposase N-terminus and the need for asymmetric transposon ends for cellular activity. We found that phosphorylation at predicted casein kinase II sites ...[more]