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RNA seq of MLL-ENL transformed cells under serine/glycine starvation including a starvation resistant clone

ABSTRACT: RNA seq of MLL-ENL transformed cells under serine/glycine starvation including a starvation resistant clone

PROVIDER: PRJEB36459 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR3843140.fastq.gz	Fastqsanger.gz
	ERR3843141.fastq.gz	Fastqsanger.gz
	ERR3843142.fastq.gz	Fastqsanger.gz

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RNA seq of MLL-ENL transformed cells under serine/glycine starvation including a starvation resistant clone

Project description:Primary hematopoietic cells were transformed by retrovirally transduced MLL-ENL. The resulting cell population was either cultured in full medium or under starvation conditions without serine and glycine. In addition a spontaneously arising ser/gly starvation resistant clone was included in this experiment. Nascent RNA was isolated by labeling cells for 1h with 4-thio-uridine and subsequent biotinylation of labeled RNA followed by streptavidin chromatography as described in Garcia-Cuellar et al. CellReports S22111247(16)30259-5.

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The next-generation RNA and ATAC sequencing of MLL-ENL transformed cell lines reveals that C/EBP transcription factors coordinate the expression of the MLL-ENL/Hoxa target genes [ATAC-seq]

Project description:We report the application of next-generation RNA and ATAC sequencing technology for high-throughput profiling of MLL-ENL transformed cell lines. According to our results, the C/EBP transcription factors coordinate the expression of the MLL-ENL/Hoxa target genes. Genetic removal of both Cebpa and Cebpb revealed a surrogate function of C/EBPε for myeloid MLL-ENL transformation.

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The next-generation RNA and ATAC sequencing of MLL-ENL transformed cell lines reveals that C/EBP transcription factors coordinate the expression of the MLL-ENL/Hoxa target genes [RNA-seq]

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RNA-Seq of human AML samples, and human cells transformed with MLL-ENL or MLL-AF6

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Epigenomic changes following acute serine starvation in breast cancer cells

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