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RNA and miRNA sequencing of extracellular vesicles derived from murine Th1 and Th2 differentiated cells

ABSTRACT: RNA and miRNA sequencing of extracellular vesicles derived from murine Th1 and Th2 differentiated cells

PROVIDER: PRJEB36937 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR3957670.fastq.gz	Fastqsanger.gz
	ERR3957671.fastq.gz	Fastqsanger.gz
	ERR3957672.fastq.gz	Fastqsanger.gz
	ERR3957673.fastq.gz	Fastqsanger.gz
	ERR3957674.fastq.gz	Fastqsanger.gz

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RNA and miRNA sequencing of extracellular vesicles derived from murine Th1 and Th2 differentiated cells

Project description:T helper cells produce extracellular vesicles. These vesicles have the ability to enter naive B cells. In our work we characterized protein, RNA and miRNA content. In this experiment, we sequenced RNA which is larger than 200bp and lower than 200bp to discriminate and enrich for long RNA products such as mRNA, lnRNA and miRNA.

2022-05-06 | E-MTAB-8823 | biostudies-arrayexpress

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Rna-seq of murine B cells to extracellular vesicles derived from Th1 and Th2 differentiated cells under resting or stimulating conditions with or without NFKB inhibitor.

Project description:Exploring the biological properties of extracellular vesicles derived from T helper cells and their influence on naive B cells.

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Rna-seq of murine B cells to extracellular vesicles derived from Th1 and Th2 differentiated cells under resting or stimulating conditions with or without NFKB inhibitor.

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Impact of Group Psychological Interventions on Extracellular Vesicles in People Who Had Cancer

Project description:Background: There is some evidence demonstrating the effect of psychological interventions in improvements in health biological parameters. To best of our knowledge, no study had addressed the impact of any psychological intervention on extracellular vesicles. In addition, Mindfulness-Based Cognitive Therapy (MBCT) and Emotion Focused Therapy for Cancer Recovery (EFT-CR) in the group have never been explored regarding extracellular vesicles and the effectiveness of these was not compared yet. Objectives: 1. To explore and compare the effect of MBCT and EFT-CR on biological parameters and psychological variables in distressed people who have had breast, prostate and colorectal cancer; 2. In addition, we will explore the acceptability through recruitment and retention rates of MBCT and EFT-CR in group and evaluate whether these interventions are appropriate for a larger clinical trial. Methods: The design of this study is a parallel randomized controlled trial. Participants will be randomized into MBCT, EFT-CR or usual care. Outcome measures will be assessed before, at the end of the intervention (8 weeks) and follow-ups (24 and 52 weeks from the baseline moment). Hypotheses: The researchers expected that both interventions will have an effect on extracellular vesicles and other study biomarkers as well as improvements in psychological outcomes, compared to treatment as usual (TAU) group. Regarding the comparative effectiveness, we did not have evidence to hypothesize which one of the interventions will be superior in both biological (extracellular vesicles) and psychological outcomes. Contribution for practice: The results of this preliminary study would permit to know if there are benefits of these psychological interventions on changes in extracellular vesicles and on psychological outcomes related to health. In addition, this study will permit to determine the acceptability of conducting a larger randomized controlled trial.

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Expression data from human Th1 and Th2 cells

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