Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Intrapatient interspecific transmission of mcr-1-carrying plasmid and other plasmidal transmission cases

ABSTRACT: Study of plasmidal transmissions of mcr-1 in a large tertiary hospital in the Netherlands

PROVIDER: PRJEB37819 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

IncHI2 mcr-1 Plasmid

Project description:mcr-1 outbreak on swiss Pigfarm

| PRJEB45636 | ENA

Gene expression microarray data by Moutan Cortex Radicis (MCR) in the LPS-stimulated human gingival fibroblast

2012-06-28 | GSE38977 | GEO

MCR sequencing

Project description:Sequences of MCR postive isolates from veterinary samples in the Netherlands.

| PRJEB29116 | ENA

Fitness cost of a mcr-1-carrying IncHI2 plasmid.

Project description:IncHI2 is a common type of large mcr-1-carrying plasmids that have been found worldwide. Large plasmids could impose metabolic burden for host bacterial strains, we therefore examine the stability and fitness cost of a mcr-1-carrying 265.5-kb IncHI2 plasmid, pMCR1_1943, in Escherichia coli in nutrient-rich LB and nutrient-restricted M9 broth. Stability tests revealed that pMCR1_1943 was stably maintained with a stability frequency of 0.99±0.01 (mean ± standard deviation) after 880 generations in LB and 0.97±0.00 after 220 generations in M9 broth. Relative fitness (expressed as w, defined as relative fitness of the plasmid-carrying strain compared to the plasmid-free progenitor strain) was examined using the 24-h head to head competitions. pMCR1_1943 initially imposed costs (w, 0.88±0.03 in LB, 0.87±0.01 in M9) but such costs were largely reduced after 14-day cultures (w, 0.97±0.03 in LB, 0.95±0.03 in M9). The stable maintenance and the largely compensated cost after passage may contribute to the wide spread of mcr-1-carrying IncHI2 plasmids. To investigate potential mechanisms for the reduced fitness cost, we performed whole genome sequencing and single nucleotide polymorphism calling for the competitor strains. We identified that molecular chaperone-encoding dnaK, cell division protein-encoding cpoB and repeat protein-encoding rhsC were associated with the cost reduction for pMCR1_1943, which may represent new mechanisms for host bacterial strains to compensate fitness costs imposed by large plasmids and warrant further studies.

| S-EPMC6306219 | biostudies-literature

mcr-1

Project description:Detection of mcr-1 gene in ESBL-E from the Netherlands, 2009-2015

| PRJEB12288 | ENA

Gene expression microarray data by Moutan Cortex Radicis (MCR) in the LPS-stimulated human gingival fibroblast

Project description:The Moutan Cortex Radicis (MCR) has been used as an analgesic, sedative and anti-inflammatory agent. This study investigated the changes in gene expression by MCR treatment when stimulated with lipopolysaccharide (LPS) in cultured human gingival fibroblasts (HGFs) and the gene expression changes by the MCR when challenged with LPS using a microarray chip. Human gingival fibroblast were divided into three experimental groups; 1, C: Control, 2, LPS: LPS-treatment only, 3, MCR40: LPS- and MCR40-treatments. Total RNA was isolated from each experimental fibroblast (3 experimental group M-CM-^W 1 sample of each experimental group = total 3 samples).

2012-06-27 | E-GEOD-38977 | biostudies-arrayexpress

mcr-1-carrying Enterobacteriaceae from chicken meat

Project description:Occurrence of mcr-1-carrying Enterobacteriaceae from chicken meat supplied from Portuguese farms

| PRJEB44904 | ENA

mcr-SFG

Project description:mcr isolates from seafoods

| PRJEB79149 | ENA

IncP Plasmid Carrying Colistin Resistance Gene mcr-1 in Klebsiella pneumoniae from Hospital Sewage.

Project description:A Klebsiella pneumoniae strain of sequence type 313 (ST313) recovered from hospital sewage was found carrying the plasmid-borne colistin resistance gene mcr-1, which was bracketed by two copies of the insertion sequence ISApl1 on a 57-kb self-transmissible IncP-type plasmid of a new IncP-1 clade. The carriage of mcr-1 on a self-transmissible broad-host-range plasmid highlights that mcr-1 has the potential to spread beyond the Enterobacteriaceae family.

| S-EPMC5278755 | biostudies-literature

MCR-4 in The Netherlands

Project description:Appearance of rare mcr-4 encoding plasmids in humans and animals in The Netherlands

| PRJEB45740 | ENA