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Zebrafish il-4 il-10 regulated immunity in gills

ABSTRACT: Zebrafish il-4 il-10 regulated immunity in gills

PROVIDER: PRJEB37851 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR4029240_1.fastq.gz	Fastqsanger.gz
	ERR4029240_2.fastq.gz	Fastqsanger.gz
	ERR4029250_1.fastq.gz	Fastqsanger.gz
	ERR4029250_2.fastq.gz	Fastqsanger.gz
	ERR4029241_1.fastq.gz	Fastqsanger.gz

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Zebrafish il-4 il-10 regulated immunity in gills

Project description:The evolutionary origin of vertebrate type 2 immunity is a topic of great interest. Several studies have focused on the immune cell components of evolutionary older vertebrates such as fish. However, how fish cytokines function and whether they have similar roles as in mammals is still a matter of speculation. Here, we have used the zebrafish (Danio rerio) to gain insights into il4/13a and il4/13b genes and characterized their role under both homeostatic and inflammatory conditions. We established knockouts for both il4/13a and il4/13b genes and showed that they are needed to suppress inflammation in larvae and in the gill mucosa. As a counterpoint, we examined the gills of il10 defective zebrafish and revealed the importance of il10 in maintaining homeostasis in this mucosal tissue. As in mammals, zebrafish il10 appears to have an anti-inflammatory function.

2020-05-28 | E-MTAB-8958 | biostudies-arrayexpress

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Project description:Zebrafish il-4 il-10 regulated immunity in gills

2023-06-16 | E-MTAB-8958 | ExpressionAtlas

IL-10 dampens antitumor immunity and promotes liver metastasis via PD-L1 induction

Project description:Background & Aims: The liver is one of the organs most commonly affected by metastasis. The presence of liver metastasis is reported to be responsible for an immunosuppressive microenvironment and diminished immunotherapy efficacy. Here, we aimed to investigate the role of IL-10 in liver metastasis formation and decipher its therapeutic potential in affecting immunotherapy effectiveness. Methods: To induce spontaneous or forced liver metastasis in mice, murine cancer cells (MC38) or colon tumor organoids were injected into the cecum or the spleen, respectively. Mice with complete and cell-type specific deletion of IL-10- and IL-10Ra were used to identify the source and the target of IL-10 during metastasis formation. Programmed death ligand 1 (PD-L1)-deficient mice were used to test the role of this checkpoint. Flow cytometry was applied to characterize the regulation of IL-10 on PD-L1. Results: We found that Il10-deficient mice and mice treated with IL-10Ra antibodies were protected against liver metastasis formation. Furthermore, by using IL-10 reporter mice, we demonstrated that Foxp3+ regulatory T cells (Tregs) were the major cellular source of IL-10 in liver metastatic sites. Accordingly, deletion of IL-10 in Tregs, but not in myeloid cells, led to reduced liver metastasis. Mechanistically, IL-10 acted on Tregs in an autocrine manner, thereby further amplifying IL-10 production. Furthermore, IL-10 acted on myeloid cells, i.e., monocytes, and induced the upregulation of the immune checkpoint protein PD-L1. Finally, the PD-L1/PD-1 axis attenuated CD8-dependent cytotoxicity against metastatic lesions. Conclusions: Treg-derived IL-10 upregulates PD-L1 expression in monocytes, which in turn reduces CD8+ T-cell infiltration and related antitumor immunity in the context of colorectal cancer (CRC)-derived liver metastasis. These findings provide the basis for future monitoring and targeting of IL-10 in CRC-derived liver metastasis.

2024-02-09 | GSE247304 | GEO

CD8+ T cells: IL-10+ VS IL-10-

Project description:Transcriptional profiling and gene expression profiling analysis of sorted CD8+IL-10+ T cells compared to CD8+IL-10- T cells using IL-10-GFP(tiger) reporter mice

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The gills transcriptome analysis of yellow catfish (Tachysurus fulvidraco♀ × P. vachellii♂) under transport stress

Project description:Transport stress is a big threat to most teleost fish during production practice, causing mass loss to the aquaculture industry. Fish gills are mucosa-associated lymphoid tissue that directly contacted with water, which is the good choice to study the mechanism of transport stress. Gills transcriptome results revealed 1551 differentially expressed genes (744 up-regulated and 807 down- regulated) between the control and 16h transportation groups. The top 10 enriched KEGG pathways are all immune-related. After qRT-PCR validation, we found that the toll-like receptors and nod-like receptors signaling pathways mediate the gill’s immune response to transport stress: tlr5, tnfɑ, hsp90ɑ, il-1ß and map2k4 were significantly up-regulated and arrdc2 was significantly down-regulated under transport stress. These results suggested that the transport stress altered innate immunity responses and induced metabolic changes in gills of hybrid yellow catfish. In conclusion, the results provide new perspectives on immune response in yellow catfish against transport stress and provide the possibility of mining resistance genes for future optimize transportation plan.

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2013-06-21 | E-GEOD-37739 | biostudies-arrayexpress

microRNA expression data from nucleated cells excluding tumor cells in malignant pleural effusion (MPE) from WT and IL-10 knock out (IL-10-/-)

Project description:The interactions between tumor cells and the host vasculature and immune cells results in increased net fluid production via enhanced plasma extravasation into the pleural space.Understanding the regulatory mechanism of IL-10 in MPE formation is essential both for deciphering how IL-10 acts in regulating tumor immunity and for discovering key molecular targets for intervention in advanced cancers. We investigate the characteristic of microRNAs (miRNAs) in IL-10 induced murine MPE. Identifing the distinct classes of up-regulated and down-regulated miRNAs during this process.

2019-08-19 | GSE135939 | GEO

IL-10 dampens antitumor immunity and promotes liver metastasis via PD-L1 induction

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