ABSTRACT: Genomic analysis of sequential outbreaks of Burkholderia cepacia and multi-drug resistant extended spectrum beta-lactamase producing Klebsiella species in a West African tertiary hospital neonatal unit
Project description:Burkholderia cenocepacia J2315 belongs to the ET12 lineage which has caused outbreaks among cystic fibrosis patients worldwide. Strains belonging to the ET12 lineage are characterized by the presence of the “Burkholderia cepacia epidemic strain marker” (BCESM). This marker is located on a genomic island involved in virulence, but the exact function of many genes in the BCESM region remains unknown. In the present study we investigated the function of three of these genes (BCAM0257, BCAM0258 and BCAM0259). We created overexpression mutants and performed a transcriptome analysis of the toxin (BCAM0258) overexpression mutant.
Project description:Extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae: Insights from a tertiary hospital in Southern Thailand
Project description:The present study examines changes in global gene expression patterns and in virulence factor-associated genes in an extended spectrum beta-lactamase (ESBL)-producing UPEC (ESBL019) during the morphologic transitions induced by an ineffective antibiotic and in the presence of human primary bladder epithelial cells. The morphological shifts induced by ineffective antibiotics are associated with significant transcriptional virulence alterations in ESBL-producing UPEC, which may affect survival and persistence in the urinary tract.
2017-06-06 | GSE99661 | GEO
Project description:Evolution of extended-spectrum beta-lactamase-producing Escherichia coli
Project description:Whole-genome sequencing revealed independent emergence of vancomycin-resistant Enterococcus faecium causing sequential outbreaks over three years in a tertiary care hospital
