Project description:In order to understand the genomic and transcriptomic variability of the axolotl pallium, as well as reconstruct their intrinsic gene regulatory networks, we performed single-nucleus multiome sequencing (RNA and open chromatin) of whole axolotl pallium.

2022-07-20 | E-MTAB-11666 | biostudies-arrayexpress

Single Cell RNA / Single Nucleus RNA Sequencing Data of Human Pancreaitc Islet

Project description:Single Cell RNA / Single Nucleus RNA Sequencing Data of Human Pancreaitc Islet

| PRJNA900882 | ENA

Single nucleus RNA sequencing of human iPSC-derived forebrain organoids to assay cell type composition

Project description:Human iPSC line D1 was differentiated into spheroids. After 21 weeks in culture, single nucei were extracted and assayed using single nucleus RNA sequencing to characterize the spheroids' cell type composition.

2021-12-01 | E-MTAB-10605 | biostudies-arrayexpress

Single-nucleus and single-cell RNA-sequencing of human pancreatic islets

Project description:Transcriptomics is frequently used to interrogate alterations in cultured human islet cells using single-cell RNA-sequencing. We introduce single-nucleus RNA-sequencing as an alternative approach for investigating human islets.

2022-07-10 | GSE207632 | GEO

Single nucleus RNA sequencing in mouse brown adipose tissue at room temperature, cold condition and thermoneutrality

Project description:Single nucleus RNA sequencing in mouse brown adipose tissue at room temperature, cold condition and thermoneutrality.

2020-07-13 | E-MTAB-8562 | biostudies-arrayexpress

Single-nucleus total RNA-sequencing of C2C12 myoblasts via in situ polyadenylation

Project description:Current methods for single-nucleus RNA-sequencing (snRNAseq) are limited to assaying endogenously polyadenylated (A-tailed) RNA transcripts. Here we demonstrate that enzymatic in situ polyadenylation of RNAs enables detection of the full spectrum of RNAs, expanding the scope of sequencing-based single-nucleus transcriptomics to the total transcriptome. We apply standard snRNAseq and total snRNAseq to C2C12 myoblasts. Our in situ polyadenylation strategy relies on a brief, low-cost add-on to a widely used protocol for single-cell/single-nucleus RNA-sequencing, and thus could be broadly and quickly adopted. Single-nucleus RNA-sequencing of the total transcriptome will enable new insights into gene regulation and biology.

2022-08-01 | GSE209780 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data