Project description:We got insights into the B. bifidum PRL2010 genes whose expression resulted to be affected when bacterial cells were cultivated on kefir and kefiran as the unique carbon source. In order to exploit the transcriptome of PRL2010 grown on kefir and hefiran we performed global transcription profiling using PRL2010 microarrays hybridized with cDNA from the RNA samples of B. bifidum PRL2010 cultivated on these substrates. We isolated mRNA from B. bifidum PRL2010 cells collected from a culture of kefir grains and from PRL2010 cultivated on MRS plus kefiran at upon 12 hours following inoculation. Microarray analysis was performed with an oligonucleotide array based on the B. bifidum PRL2010 genome: a total of 8,130 oligonucleotide probes of 60bp in length were designed on 1707 ORFs using eArray5.0 (Agilent Technologies). 5 Oligos were designed for each gene on a 4x44k Agilent Microarrays(Agilent Technologies, Santa Clara, CA, USA). Replicates were distributed on the chip at random, non-adjacent positions.

2016-07-03 | E-GEOD-50506 | biostudies-arrayexpress

Metagenomic regulatory sequence library characterized in RpoD ortholog strains

Project description:Characterization of a metagenomic regulatory sequence library derived from M. xanthus, E. coli, and O. urethralis genomes in strains expressing different RpoD ortholog variants. Targeted DNA and RNA seq used to profile relative DNA and RNA abundances, respectively of each regulatory sequence construct in the library.

2022-06-07 | E-MTAB-9111 | biostudies-arrayexpress

Metagenomic of Kefir-UDI strain

Project description:Microbial composition of Kefir- UDI strain using shotgun and amplicons metagenomics

| PRJEB40661 | ENA

Water Kefir

Project description:Water Kefir Metagenomes

| PRJNA977472 | ENA

Gene expression data from a cohort of Chilean Ulcerative Colitis human intestinal samples

Project description:Different genes, especially cytokines, have been deregulated in the inflammatory environment of intestinal mucosa in ulcerative colitis patients. The effects of differential gene expression such as immunological factors have been described before, however, there is no evidence of alarmins deregulated by microRNAs impacting on the pathophysiology of UC. Our goal is to study deregulated genes in inflamed mucosa for microRNA pairing in a Chilean cohort of patients. We used microarrays to compare inflamed and non inflamed mucosa from chilean ulcerative colitis patients

2019-10-07 | GSE133060 | GEO

Kefir CMBi

Project description:Metagenome of Chiean Kefir

| PRJEB21245 | ENA

Kefir peptide profiling - Peptidomic analysis reveals proteolytic activity of kefir microorganisms on bovine milk proteins

Project description:This study examines the proteolytic activity of the kefir grains (a combination of bacteria and yeast) on bovine milk proteins. SDS-PAGE analysis reveals substantial digestion of milk proteins by the kefir grains in comparison with control samples. Mass spectrometric analysis reveals that the kefir microorganisms released 609 new peptide fragments and significantly altered the abundance of around 1,500 peptides compared to the controls. These kefir-digested peptides derived from 55 milk proteins. We show that kefir contains 25 previously identified functional peptides with actions including antihypertensive, antimicrobial, opioid and anti-oxidative .

2016-05-09 | PXD001826 | Pride

Chilean vineyards

Project description:ITS2 seqeuncing of 34 chilean vineyards

| PRJEB75257 | ENA