12 quinolone-resistant Escherichia coli
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PROVIDER: PRJEB40078 | ENA |
REPOSITORIES: ENA
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Project description:Nanopore sequence data for 12 quinolone-resistant Escherichia coli containing qnrS1
| PRJEB40268 | ENA
Project description:Global response to ciprofloxacin in low level quinolone resistant Escherichia coli: a shorter path to survival. Background: Bactericidal activity of quinolones in bacteria has been related to a combination of DNA fragmentation, ROS production and programmed dead cell systems. Subjacent molecular systems responsible for reduction of bactericidal effect in low-level quinolone resistance (LLQR) phenotypes remain to be clarified. To answer this question and to define new possible antimicrobial targets, the transcriptomic profile in isogenic Escherichia coli harbouring quinolone resistance mechanisms in the presence of ciprofloxacin was evaluated. Materials and methods: E. coli 25922 was used as background strain. Four LLQR isogenic strains were tested for transcriptomic assays: ATCC 25922 (wild-type), EC14 (coding for QnrS1), EC19 (marR deletion and coding for QnrS1) and EC24 (Ser83Leu substitution in GyrA and coding for QnrS1). Cells in exponential phase (DO600=0.4) were exposed to 1 mg/L of ciprofloxacin (breakpoint for reduced susceptibility according to CLSI) during 1 hour and, further, RNA was purified. Gene expression analysis was performed using AGILENT technology. Data obtained for each strain were always normalized to the wild-type E. coli ATCC 25922. Specific ROS modulation targets were validated by genetic and biochemical approach. Results: A radical differential response to ciprofloxacin in LLQR strains, either up or downregulation, was observed (proportional to the MIC values). Multiple genes implicated in ROS production (related to TCA cycle, aerobic respiration or detoxification systems) were upregulated (sdhC up to 63.5-folds) in LLQR mutants. SOS system components were downregulated (recA up to 30.7-folds). yihE, coding for a protective kinase of programmed cell death, was also upregulated (up to 5.2-folds). SdhC inhibition sensitized LLQR phenotypes (up to Log=2.3 after 24 hours). Conclusions: At clinical relevant concentration of ciprofloxacin, the pattern of genes expression of critical systems for bacterial survival and mutant development were significantly modified in LLQR phenotypes. This approach allowed validating ROS modulation as an interesting target in terms of bacterial sensitization.
2017-01-01 | GSE86341 | GEO
Project description:Meningococcal quinolone resistance originated from several commensal Neisseria species
| PRJEB35093 | ENA
Project description:Hybrid assemblies of E. coli with plasmid-mediated quinolone resistance genes
| PRJEB43438 | ENA
Project description:In total, 12 quinolone-resistant Escherichia coli (QREC) strains containing qnrS1 were submitted to long-read sequencing using a FLO-MIN106 flow cell on a MinION device. The long reads were assembled with short reads (Illumina) and analyzed using the MOB-suite pipeline. Six of these QREC genome sequences were closed after hybrid assembly.
| S-EPMC7844070 | biostudies-literature
Project description:Escherichia coli BW25113 strain K-12 containing pHNSHP45 plasmid Raw sequence reads
| PRJNA1068323 | ENA
Project description:In order to determine how the bacterial quorum-sensing precursor molecule 2-heptyl-4-quinolone (HHQ) induces cellular senescence in populations of some eukaryotic marine phytoplankton, we examined the transcriptional response of Emiliania huxleyi CCMP2090 to HHQ exposure. Quadruplicate batch cultures were treated with HHQ at three concentrations (1, 10 and 100 ng/ml) and compared to a vehicle control after 24 and 72 hours of exposure. The majority of gene expression changes occurred after 24 hr of exposure in the 10 and 100 ng/ml treatments and many of these changes persisted for 72 hr in the 100 ng/ml treatment. RNAseq analysis revealed that HHQ exposure impacts many aspects of Emiliania huxleyi metabolism including: chlorophyll biosynthesis and light harvesting, vitamin biosynthesis, vesicle trafficking, and general signals related to energy production. Changes in genes involved in intraphase cell cycle check points implicate DNA damage as the primary driver of HHQ-induced cellular senescence.
2020-07-30 | GSE131846 | GEO
Project description:Draft Genome Sequences of Aeropyrum pernix YK1-12-2013
| PRJDB5509 | ENA