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Agrin-MEC

ABSTRACT: Temporal response of soil bacterial communities to application of organic and inorganic phosphate based fertilisers under of Vicia faba L cultivation

PROVIDER: PRJEB40215 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR4567530_1.fastq.gz	Fastqsanger.gz
	ERR4567530_2.fastq.gz	Fastqsanger.gz
	ERR4567540_1.fastq.gz	Fastqsanger.gz
	ERR4567540_2.fastq.gz	Fastqsanger.gz
	ERR4567550_1.fastq.gz	Fastqsanger.gz

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Organic phosphate but not inorganic phosphate regulates Fgf23 expression through MAPK and TGF-ꞵ signaling

Project description:One of the main regulators of phosphate homeostasis is fibroblast growth factor 23 (FGF23), secreted by osteocytes. The effects of organic versus inorganic dietary phosphate on this homeostasis is unclear. This study used MC3T3-E1 osteocyte-like cells to examine the transcriptomic responses to these phosphates. Most importantly, the expression and secretion of FGF23 was only increased in response to organic phosphate. Gene ontology terms related to a response to environmental change were only enriched in osteocytes treated with organic phosphate while osteocytes treated with inorganic phosphate were enriched for terms associated with regulation of cellular phosphate metabolism. Inhibition of MAPK signaling diminished the response of Fgf23 to organic phosphate, suggesting it activates FGF23. TGF-β signaling inhibition increased Fgf23 expression after the addition of organic phosphate, while the negative TGF-β regulator Skil decreased this response. In summary, the observed differential response of osteocytes to phosphate types may have consequences for phosphate homeostasis.

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Transcription profiling of developing pea cotyledons over-expressing VfAAP1

Project description:Nitrogen application to legume seeds regulates seed metabolism and composition. In order to improve nitrogen flux into the embryo, the Vicia faba amino acid permease VfAAP1 (Miranda et al. Amino acid permeases in developing seeds of Vicia faba L.: expression precedes storage protein synthesis and is regulated by amino acid supply. Plant J 2001 28: 61-72) was expressed in pea under control of the seed-specific LeB4 promotor (Bäumlein et al. Cis-analysis of a seed protein gene promoter: the conservative RY repeat CATGCATG within legumin box is essential for tissue-specific expression of a legumin gene. Plant J 1992 2: 233-239). Several transgenic lines have been generated. Mature seeds of the homozygous marker gene-free line AAP14/10 showed an increase in amino acid supply, seed nitrogen and protein content due to higher globulin fraction. The effect of VfAAP1 was tested under field conditions in two growing periods, 2005 and 2006, and the data could be confirmed. Over-expression of VfAAP1 interferes with storage protein metabolism and alters fluxes of nitrogen during seed growth. The influence of VfAAP1 on altered gene expression in developing cotyledons was analysed using a 6k-Oligo-microarray. Four developmental stages (18, 22, 26 and 30 DAP) from seeds, grown 2006, of the transgenic line AAP14/10 were hybridized against wildtype control.

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Project description:Symbiotic nitroegn fixation in functional (Fix+) and non-functional (Fix-) nodules of Vicia faba infected with Rhizobium leguminosarum was investigated using label-free shotgun tandem MS. Proteins involved in symbiotic nitrogen fixation and maintenance of the symbiosis were identified.

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