MAGs of uncultured fungal symbionts in a lichen symbiosis
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB40332 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| Other | ||||
| CAJHEP01.dat.gz | Other | |||
| CAJHEP01.fasta.gz | Fasta.gz | |||
| CAJHEP01.master.dat | Other | |||
| CAJHEQ01.dat.gz | Other |
Items per page: 5 1 - 5 of 19 |
Similar Datasets
Project description:Ascomycete fungi that are symbionts of lichens from the Lecanoromycetes Genome sequencing and assembly
| PRJNA795879 | ENA
Project description:Cellular mechanisms responsible for the regulation of nutrient exchange, immune responses, and symbiont population growth in the cnidarian-dinoflagellate symbiosis are poorly resolved, particularly with respect to the dinoflagellate symbiont. Here, we characterised proteomic changes in the native symbiont Breviolum minutum during colonisation of its host sea anemone Exaiptasia diaphana (‘Aiptasia’). We also compared the proteome of this native symbiont in the established symbiotic state with that of a non-native symbiont, Durusdinium trenchii. The onset of symbiosis between Aiptasia and B. minutum induced increased accumulation of symbiont proteins associated with acquisition of inorganic carbon and photosynthesis, nitrogen metabolism, micro- and macronutrient starvation, suppression of the host immune responses, tolerance to low pH, and management of oxidative stress. Such responses are consistent with a functional, persistent symbiosis. In contrast, D. trenchii predominantly showed elevated levels of immunosuppressive proteins, consistent with the view that this symbiont is an opportunist that forms a less beneficial, less well-integrated symbiosis with this model anemone. By adding this analyses of the symbiont proteins to the already known responses of the host proteome, our results provide a more holistic view of cellular processes that determine host-symbiont specificity and how differences in symbiont partners, native versus non-native symbionts, may impact the fitness of the cnidarian-dinoflagellate symbiosis in response to thermal stress. This PRIDE entry contains the Breviolum minutum data; Durusdinium trenchii data are uploaded in a separate entry with identical parameters.
2024-01-18 | PXD045585 | Pride
Project description:Cellular mechanisms responsible for the regulation of nutrient exchange, immune responses, and symbiont population growth in the cnidarian-dinoflagellate symbiosis are poorly resolved, particularly with respect to the dinoflagellate symbiont. Here, we characterised proteomic changes in the native symbiont Breviolum minutum during colonisation of its host sea anemone Exaiptasia diaphana (‘Aiptasia’). We also compared the proteome of this native symbiont in the established symbiotic state with that of a non-native symbiont, Durusdinium trenchii. The onset of symbiosis between Aiptasia and B. minutum induced increased accumulation of symbiont proteins associated with acquisition of inorganic carbon and photosynthesis, nitrogen metabolism, micro- and macronutrient starvation, suppression of the host immune responses, tolerance to low pH, and management of oxidative stress. Such responses are consistent with a functional, persistent symbiosis. In contrast, D. trenchii predominantly showed elevated levels of immunosuppressive proteins, consistent with the view that this symbiont is an opportunist that forms a less beneficial, less well-integrated symbiosis with this model anemone. By adding this analyses of the symbiont proteins to the already known responses of the host proteome, our results provide a more holistic view of cellular processes that determine host-symbiont specificity and how differences in symbiont partners, native versus non-native symbionts, may impact the fitness of the cnidarian-dinoflagellate symbiosis in response to thermal stress. This PRIDE entry contains the Durusdinium trenchii data; Breviolum minutum data are uploaded in a separate entry with identical parameters.
2024-01-18 | PXD045587 | Pride
Project description:The goal of this study was to use heterologous microarray hybridization to determine genomic content shared among different vesicomyid symbionts. These symbionts are closely related and can be thought of as different strains of bacteria, facilitating the use of heterologous microarray hybridization to determine genomic content. Keywords: comparative genomic hybridization Microarrays were built off the Ruthia magnifica genome and two replicate hybridizations to this organism were used as a baseline for comparisons. Genomic DNA from two other vesicomyid symbionts (Calyptogena kilmeri and C. pacifica symbionts) was also hybridized to the array with three biological replicates for each sample.
2010-05-15 | E-GEOD-13447 | biostudies-arrayexpress
Project description:The goal of this study was to use heterologous microarray hybridization to determine genomic content shared among different vesicomyid symbionts. These symbionts are closely related and can be thought of as different strains of bacteria, facilitating the use of heterologous microarray hybridization to determine genomic content. Keywords: comparative genomic hybridization
2008-11-28 | GSE13447 | GEO
Project description:The lower needs for chemical fertilisers make the Pisum Sativum well suited for sustainable agricultural systems. During the past decades, yield losses were mainly due to pathogenic infestation, with Ascochyta blight as the most severe disease. In this fungal disease complex Didymella pinodes, as the most aggressive pathogen, has been examined intensively. Despite the great number of varieties, no resistance was yet found and several strategies were developed to avoid infestation. In recent years enhanced resistance to pathogens due to previous infestation by symbiotic microorganisms was discovered in several plants. However, only little attention was drawn on the implications of P. Sativum´s symbionts (Rhizobia, Mycorrhiza) on the disease severity as well as the effects of the pathogen on the symbiotic interactions. Hence, here the implications of the pathogenic infestation on the tripartite symbiosis of P. Sativum on a molecular (proteomics, metabolomics) and physiological level, and moreover, the potential of the root symbionts to enhance resistance against Didymella pinodes are investigated.
2017-01-16 | PXD005700 | Pride
Project description:The lower needs for chemical fertilisers make the Pisum Sativum well suited for sustainable agricultural systems. During the past decades, yield losses were mainly due to pathogenic infestation, with Ascochyta blight as the most severe disease. In this fungal disease complex Didymella pinodes, as the most aggressive pathogen, has been examined intensively. Despite the great number of varieties, no resistance was yet found and several strategies were developed to avoid infestation. In recent years enhanced resistance to pathogens due to previous infestation by symbiotic microorganisms was discovered in several plants. However, only little attention was drawn on the implications of P. Sativum´s symbionts (Rhizobia, Mycorrhiza) on the disease severity as well as the effects of the pathogen on the symbiotic interactions. Hence, here the implications of the pathogenic infestation on the tripartite symbiosis of P. Sativum on a molecular (proteomics, metabolomics) and physiological level, and moreover, the potential of the root symbionts to enhance resistance against Didymella pinodes are investigated.
2017-01-16 | PXD005701 | Pride
Project description:We compared the transcriptomes of tissues from Oral lichen planus patients with immunosuppressive therapy to reveal the biological mechanism of oral lichen planus treatment.
2022-06-05 | GSE204663 | GEO
Project description:Basidiomycete yeasts have recently been reported as stably associated secondary fungal symbionts of many lichens, but their role in the symbiosis remains unknown. Attempts to sequence their genomes have been hampered both by the inability to culture them and their low abundance in the lichen thallus alongside two dominant eukaryotes (an ascomycete fungus and chlorophyte alga). Using the lichen Alectoria sarmentosa, we selectively dissolved the cortex layer in which secondary fungal symbionts are embedded to enrich yeast cell abundance and sequenced DNA from the resulting slurries as well as bulk lichen thallus. In addition to yielding a near-complete genome of the filamentous ascomycete using both methods, metagenomes from cortex slurries yielded a 36- to 84-fold increase in coverage and near-complete genomes for two basidiomycete species, members of the classes Cystobasidiomycetes and Tremellomycetes. The ascomycete possesses the largest gene repertoire of the three. It is enriched in proteases often associated with pathogenicity and harbors the majority of predicted secondary metabolite clusters. The basidiomycete genomes possess ∼35% fewer predicted genes than the ascomycete and have reduced secretomes even compared with close relatives, while exhibiting signs of nutrient limitation and scavenging. Furthermore, both basidiomycetes are enriched in genes coding for enzymes producing secreted acidic polysaccharides, representing a potential contribution to the shared extracellular matrix. All three fungi retain genes involved in dimorphic switching, despite the ascomycete not being known to possess a yeast stage. The basidiomycete genomes are an important new resource for exploration of lifestyle and function in fungal-fungal interactions in lichen symbioses.
| S-EPMC8355462 | biostudies-literature