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4C-Seq identify the interaction between miR-146a promoter and rs2431697-containing region

ABSTRACT: 4C-Seq identify the interaction between miR-146a promoter and rs2431697-containing region

PROVIDER: PRJEB40393 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR4603366_1.fastq.gz	Fastqsanger.gz
	ERR4603366_2.fastq.gz	Fastqsanger.gz
	ERR4603367_1.fastq.gz	Fastqsanger.gz
	ERR4603367_2.fastq.gz	Fastqsanger.gz
	ERR4603368_1.fastq.gz	Fastqsanger.gz

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4C-Seq identify the interaction between miR-146a promoter and rs2431697-containing region

Project description:To test the genomic region containing rs2431697 whether forms a cognate enhancer-promoter loop with the miR-146a promoter and modulates the expression of miR-146a, we perfomed 4C-seq in U-937 cell line based on the rs2431697 view point and miR-146a promoter view point.

2020-11-05 | E-MTAB-8974 | biostudies-arrayexpress

4C-Seq identify the interaction between miR-146a promoter and rs2431697-containing region

Project description:To test the genomic region containing rs2431697 whether forms a cognate enhancer-promoter loop with the miR-146a promoter and modulates the expression of miR-146a, we perfomed 4C-seq in U-937 cell line based on miR-146a promoter view point (First digestion enzyme MboI, second digestion enzyme NlaIII) and rs2431697 view point (First digestion enzyme CSP6I, second digestion enzyme NlaIII).

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4C-Seq identify the interaction between miR-146a promoter and rs2431697-containing region

Project description:4C-Seq identify the interaction between miR-146a promoter and rs2431697-containing region

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4C-Seq identifies the chromatin interaction landscape of the miR-146a promoter region

Project description:To test the chromatin interaction landscape of the miR-146a promoter region, we performed 4C-seq in JURKAT and RAJI cell lines based on the miR-146a promoter viewpoint. (First digestion enzyme MboI, second digestion enzyme NlaIII)

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Project description:To test if there is a physical interaction between the IRF8 promoter and the rs2280381-containing region, we conducted circular chromatin conformation capture assay in the U937 cell. For the IRF8 viewpoint group, we used CSP6I as the first digested enzyme and NlaIII as the second enzyme. For the rs2280381-containing region, we used MboI as the first digested enzyme and NlaIII as the second enzyme. We use 4C-PCR primer to construct IRF8 point view and rs2280381-containing region 4C library.

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Project description:To test if lncRNA AC092723.1 play a role in physical interaction between the IRF8 promoter and the rs2280381-containing region, we conducted circular chromatin conformation capture assay in the human primary monocyte. We knock down lncRNA AC092723.1 by electro-transfecting ASOs, the control group is transfected negtive ASOs with no impact on lncRNA AC092723.1.We used CSP6I as the first digested enzyme and NlaIII as the second enzyme. After constructing 4C library,we then utilize 4C-PCR primer to construct IRF8 point view 4C library.

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