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Monitor the efficiency of "WIND: A Workflow for pIRNAs aNd beyonD" for the identification of piRNA in mouse samples

ABSTRACT: Monitor the efficiency of "WIND: A Workflow for pIRNAs aNd beyonD" for the identification of piRNA in mouse samples

PROVIDER: PRJEB41807 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR4969785.fastq.gz	Fastqsanger.gz
	ERR4969786.fastq.gz	Fastqsanger.gz

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Monitor the efficiency of "WIND: A Workflow for pIRNAs aNd beyonD" for the identification of piRNA in mouse samples

Project description:For the evaluation of "WIND: A Workflow for pIRNAs aNd beyonD" performance and the transcriptomic approach on small-RNA identification, and particularly on piRNAs, two mouse Adult Cardiomyocytes (aCMs) were used.

2020-11-28 | E-MTAB-9866 | biostudies-arrayexpress

Monitor the efficiency of "WIND: A Workflow for pIRNAs aNd beyonD" for the identification of piRNA molecules in small RNA-seq

Project description:WIND: A Workflow for pIRNAs aNd beyonD, for the evaluation of the performance in piRNAs high expression and low expression conditions, we used Human Testis RNAs (BioChain Institute Inc, Newark, CA, USA) and COLO 205 cell line RNAs (samples are available on ArrayExpress (E-MTAB-8115: Non_treated_Testis_1 and Non_treated_COLO205_1, Non_treated_COLO205_2, Non_treated_COLO205_3).

2020-11-25 | E-MTAB-9782 | biostudies-arrayexpress

Monitor the efficiency of "WIND: A Workflow for pIRNAs aNd beyonD" for the identification of single-stranded (SS) spike-in piRNA-like molecules in smallRNA-seq

Project description:For the evaluation of \\"WIND: A Workflow for pIRNAs aNd beyonD\\" performance and the transcriptomic approach on small-RNA identification, and particularly on piRNAs, a synthetic set of 4 piRNA-like molecules was used. Two non-methylated (SS-22 and SS-28) and two methylated (mSS-22 and mSS-28) of two different lengths (22 nt and 28 nt) were included. Spike-ins were chemically synthesised at Exiqon, the pool of 4 molecules was used at three different concentrations, with a final amount of 0.3 x 10^9 (dil_A), 0.3 x 10^10 (dil_B) and 0.3 x 10^11 (dil_C) molecules/ug of RNA. Library preparation and sequencing were done as previously described in Sellitto et al 2019 (doi: https://doi.org/10.3390/cells8111390)

2020-11-25 | E-MTAB-9772 | biostudies-arrayexpress

Monitor the efficiency of "WIND: A Workflow for pIRNAs aNd beyonD" for the identification of piRNA molecules in small RNA-seq

Project description:Monitor the efficiency of "WIND: A Workflow for pIRNAs aNd beyonD" for the identification of piRNA molecules in small RNA-seq

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Monitor the efficiency of "WIND: A Workflow for pIRNAs aNd beyonD" for the identification of single-stranded (SS) spike-in piRNA-like molecules in smallRNA-seq

Project description:Monitor the efficiency of "WIND: A Workflow for pIRNAs aNd beyonD" for the identification of single-stranded (SS) spike-in piRNA-like molecules in smallRNA-seq

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The quantitative biotinylproteomics studies reveals WInd-Related Kinase (WIRK1) functioning as a wind signaling component in Wind-induced Thigmomorphpgenesis and Gravitropism

Project description:Wind is one of the most prevalent environmental forces entraining plants to develop various mechano-responses, collectively called thigmomorphogenesis. Largely unknown is how plants transduce the complex wind force signals downstream to nuclear events and the development of thigmomorphogenic phenotype or anemotropic response. To identify molecular components of the wind drag force signaling, two force-regulated phosphoproteins, identified from our previous phosphoproteomic study of Arabidopsis touch response, mitogen-activated protein kinase kinase 1 (MKK1) and 2 (MKK2), were selected for performing in planta TurboID (ID)-based proximity-labeling (PL) proteomics. This quantitative biotinylproteomics was separately performed on MKK1-ID and MKK2-ID transgenic plants, respectively, using the TurboID overexpression transgenics as a universal control. This quantitative biotinylproteomic work successfully identified 11 and 71 MKK1- and MKK2 - associated proteins, respectively. A WInd-Related Kinase 1 (WIRK1), previously known as Rapidly Accelerated Fibrosarcoma 36 protein (RAF36), was eventually found to be a common interactor for both MKK1 and MKK2 kinases. Further molecular biology studies of the Arabidopsis RAF36 kinase found that it plays a role in wind regulation of the expression of touch-responsive TCH3 and CML38 genes and the phosphorylation of a touch-regulated PATL3 phosphoprotein. Measurement of leaf morphology and shoot gravitropic response of wirk1-1 mutant revealed that the WIRK1 gene is involved in both wind response and gravitropism of Arabidopsis, suggesting that WIRK1 protein may serve as the crosstalk point among multiple signal transduction pathways of both gravitropic and wind responses. It is likely that gravity force signaling may be an integral part of the wind mechano-signaling network in various parts of plant organs.

2024-06-22 | PXD048765 | Pride

WIND (Workflow for pIRNAs aNd beyonD): a strategy for in-depth analysis of small RNA-seq data [version 3; peer review: 2 approved]

Project description:Current bioinformatics workflows for PIWI-interacting RNA (piRNA) analysis focus primarily on germline-derived piRNAs and piRNA-clusters. Frequently, they suffer from outdated piRNA databases, questionable quantification methods, and lack of reproducibility. Often, pipelines specific to miRNA analysis are used for the piRNA research

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Wind-responsive changes in poplar leaves of different developmental stages

Project description:A developmental series of wind-treated Populus leaf tissue was subjected to array analyses in order to address the issue of age-dependent responsiveness to environmental changes. The following developmental stages were defined for the experiment: Y – “youngest leaf” including the shoot tip = smallest fully enrolled leaf; E – “expanded leaf” = oldest leaf that had not reached full leaf thickness; M – “mature leaf” = 5th leaf below E = has reached full leaf expansion and full leaf thickness; O – “old leaf” = 5th leaf below E. Keywords: transcription profiling Two-condition experiment, control (K) vs. Wind-treated (W) leaves. Biological replicates: 3 control (1-3), wind-exposed (1-3), independently grown and harvested. One swap replicate per array.

2008-04-03 | E-GEOD-11026 | biostudies-arrayexpress

Wind-responsive changes in poplar leaves of different developmental stages

2008-04-03 | GSE11026 | GEO

Maternally inherited piRNAs silence transposons during Drosophila embryogenesis

Project description:The piRNA pathway controls transposon expression in animal germ cells, thereby ensuring genome stability over generations. piRNAs are maternally deposited and required for proper transposon silencing in adult offspring. However, a long-standing question in the field is the precise function of maternally deposited piRNAs and its associated factors during embryogenesis. Here, we probe the spatio-temporal expression patterns of several piRNA pathway components during early stages of development. Amongst those, factors required for transcriptional gene silencing (TGS) showed ubiquitous abundance in somatic and pole cells throughout the first half of embryogenesis. We further analysed the transcriptomes of various embryo stages and correlated these with the presence of selected chromatin marks. We found that a number of transposon families show bursts of transcription during early embryonic stages. Transposons heavily targeted by maternally deposited piRNAs accumulated repressive chromatin marks following their spike in expression. Furthermore, depletion of maternally deposited Piwi protein in early embryos resulted in increased expression of transposons targeted by inherited piRNAs and was accompanied by a strong loss of repressive chromatin marks at coding sequences. Overall, our data suggests a pivotal role for the piRNA pathway in transposon defence during Drosophila embryogenesis in somatic cells.

2021-07-26 | PXD022409 | Pride

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