The quantitative biotinylproteomics studies reveals WInd-Related Kinase (WIRK1) functioning as a wind signaling component in Wind-induced Thigmomorphpgenesis and Gravitropism

ABSTRACT: Wind is one of the most prevalent environmental forces entraining plants to develop various mechano-responses, collectively called thigmomorphogenesis. Largely unknown is how plants transduce the complex wind force signals downstream to nuclear events and the development of thigmomorphogenic phenotype or anemotropic response. To identify molecular components of the wind drag force signaling, two force-regulated phosphoproteins, identified from our previous phosphoproteomic study of Arabidopsis touch response, mitogen-activated protein kinase kinase 1 (MKK1) and 2 (MKK2), were selected for performing in planta TurboID (ID)-based proximity-labeling (PL) proteomics. This quantitative biotinylproteomics was separately performed on MKK1-ID and MKK2-ID transgenic plants, respectively, using the TurboID overexpression transgenics as a universal control. This quantitative biotinylproteomic work successfully identified 11 and 71 MKK1- and MKK2 - associated proteins, respectively. A WInd-Related Kinase 1 (WIRK1), previously known as Rapidly Accelerated Fibrosarcoma 36 protein (RAF36), was eventually found to be a common interactor for both MKK1 and MKK2 kinases. Further molecular biology studies of the Arabidopsis RAF36 kinase found that it plays a role in wind regulation of the expression of touch-responsive TCH3 and CML38 genes and the phosphorylation of a touch-regulated PATL3 phosphoprotein. Measurement of leaf morphology and shoot gravitropic response of wirk1-1 mutant revealed that the WIRK1 gene is involved in both wind response and gravitropism of Arabidopsis, suggesting that WIRK1 protein may serve as the crosstalk point among multiple signal transduction pathways of both gravitropic and wind responses. It is likely that gravity force signaling may be an integral part of the wind mechano-signaling network in various parts of plant organs.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Whole Body

SUBMITTER: Nan Yang  

LAB HEAD: Ning Li

PROVIDER: PXD048765 | Pride | 2024-06-22

REPOSITORIES: Pride