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3’RNA-seq of post-differentiation murine CD142− and CD142+ ASPCs sorted with four different anti-CD142 antibodies

ABSTRACT: 3’RNA-seq of post-differentiation murine CD142− and CD142+ ASPCs sorted with four different anti-CD142 antibodies

PROVIDER: PRJEB43425 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR5411750.fastq.gz	Fastqsanger.gz
	ERR5411760.fastq.gz	Fastqsanger.gz
	ERR5411770.fastq.gz	Fastqsanger.gz
	ERR5411780.fastq.gz	Fastqsanger.gz
	ERR5411751.fastq.gz	Fastqsanger.gz

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3’RNA-seq of post-differentiation murine CD142− and CD142+ ASPCs sorted with four different anti-CD142 antibodies

Project description:To characterize CD142+ ASPCs (Aregs) after exposure to an adipogenic cocktail we performed bulk RNA-seq (using BRB-seq) of total, CD142− and CD142+ mouse adipose stem and progenitor cells (ASPCs), sorted using four different anti-CD142 antibodies. ASPCs were collected as Lin− (CD31− CD45− TER119−) CD29+ CD34+ SCA1+ cells of the mouse subcutaneous stromal vascular fraction using FACS.

2022-07-04 | E-MTAB-10180 | biostudies-arrayexpress

3’RNA-seq of murine CD142− and CD142+ ASPCs freshly sorted with four different anti-CD142 antibodies

Project description:To validate that the robustness of Aregs' (CD142+ ASPCs') molecular identity regardless of the antibody used, we performed transcriptomic profiling of freshly isolated CD142− and CD142+ mouse adipose stem and progenitor cells (ASPCs) sorted using four different anti-CD142 antibodies. ASPCs were collected as Lin− (CD31− CD45− TER119−) CD29+ CD34+ SCA1+ CD142+/− cells of the mouse subcutaneous stromal vascular fraction using FACS.

2022-07-04 | E-MTAB-10181 | biostudies-arrayexpress

3’RNA-seq of murine CD142− and CD142+ ASPCs freshly sorted with four different anti-CD142 antibodies

Project description:3’RNA-seq of murine CD142− and CD142+ ASPCs freshly sorted with four different anti-CD142 antibodies

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3’RNA-seq of murine CD142− ASPCs co-cultured with CD142+ ASPCs (Aregs) carrying specific gene knockdowns

Project description:To study the involvement of key Areg (CD142+ ASPC)-specifics factors in the inhibitory capacity of CD142+ ASPCs on adipogenesis, we performed transcriptomic profiling of CD142− ASPCs exposed to the secretome of CD142+ ASPCs carrying knockdowns of the indicated genes. CD142− ASPCs were co-cultured and co-differentiated (within the transwell set-up) with CD142+ ASPCs in which siRNA-based knockdowns of specific genes were performed. ASPCs were collected as Lin− (CD31− CD45− TER119−) CD29+ CD34+ SCA1+ cells of the mouse subcutaneous stromal vascular fraction using FACS.

2022-07-04 | E-MTAB-10183 | biostudies-arrayexpress

3’RNA-seq of murine post-differentiation CD142− ASPCs treated with retinoic acid (RA)

Project description:To study the impact of retinoic acid treatment on the transcriptome of CD142− ASPCs, we performed bulk RNA-seq on CD142− ASPCs treated with different RA concentrations ranging between 0.01 to 100 μM, with 100 ng/ml of BSA (negative control), DMSO (negative control) or 100 ng/ml of EGF (positive control).

2021-06-26 | E-MTAB-10184 | biostudies-arrayexpress

3’RNA-seq of murine CD142− ASPCs co-cultured with CD142+ ASPCs (Aregs) carrying specific gene knockdowns

Project description:3’RNA-seq of murine CD142− ASPCs co-cultured with CD142+ ASPCs (Aregs) carrying specific gene knockdowns

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3' RNA-seq of CD142+/- mouse adipose stem and progenitor cells

Project description:To characterize the newly discovered Areg cells, we sampled at least four replicates of mouse adipose stem and progenitor cells (ASPCs), CD142+ ASPCs and CD142- ASPCs at four distinct time-points: (1) immediately post-sort (D0), (2) after attachment (5 hours after plating, 5H), (3) after culturing (24 hours after plating, D1) and (4) after adipogenic differentiation (day 12 after plating, day 8 after induction of differentiation, D12), and investigated their transcriptome through a highly multiplexed 3 polyA-selective mRNA-sequencing assay.

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3’RNA-seq of murine fresh and expanded ASPCs

Project description:To characterize murine ASPCs we performed bulk RNA-seq (BRB-seq, Alpern et al., 2019) of total, CD142− and CD142+ (Aregs) mouse adipose stem and progenitor cells (ASPCs). ASPCs were collected as Lin− (CD31− CD45− TER119−) CD29+ CD34+ SCA1+ cells of the mouse subcutaneous stromal vascular fraction using FACS. The cells were sequenced directly after sort or after expansion.

2022-07-04 | E-MTAB-10179 | biostudies-arrayexpress

3’RNA-seq of murine ASPCs derived from P0, P16, 4wo, 7wo and 11wo-mice

Project description:To explore the molecular identity of mouse adipose stem and progenitor cells (ASPCs) and more specifically of CD142+ ASPCs (Aregs), we performed bulk RNA-seq on freshly isolated total, CD142− and CD142+ ASPCs of new-born pups (P0), post-natal day 16 mice (P16), 4 week-old (4wo), 7wo and 11wo mice. ASPCs were collected as Lin− (CD31− CD45− TER119−) CD29+CD34+ SCA1+ cells of the mouse subcutaneous stromal vascular fraction using FACS.

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Project description:To validate the anti-adipogenic effect of the newly discovered Areg cells in human, we used FACS to separate the CD142+ from the CD142- population within cultured (passage 1) adipogenic stem and progenitor cells (ASPCs) (CD31- CD45- stromal vascular fraction cells) that were derived from four individuals, and induced adipogenesis. We then performed 3 RNA-seq expression profiling of the induced cells.

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