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Gut microbiota predicts body fat change following a Low Energy Diet: a PREVIEW sub-study

ABSTRACT: PREVention of diabetes through lifestyle Intervention and population studies in Europe and around the World (PREVIEW) comprised a multi-centre, 3-year lifestyle intervention in overweight adults with pre-diabetes conducted in 8 countries, that aimed to decrease the incidence of T2D. In the present study, we study the gut microbiota from a subset of participants who followed an 8-week total meal replacement low-energy diet (LED) in the PREVIEW trial, from Finland and New Zealand (N=211), by Illumina sequencing of the 16S rRNA gene. We aimed to track changes in the composition and function of the gut microbiota during LED-induced weight loss, and to determine whether baseline microbiota configuration is associated with host metabolic response to the LED.

PROVIDER: PRJEB43667 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	ERR5470690.fastq.gz	Fastqsanger.gz
	ERR5470700.fastq.gz	Fastqsanger.gz
	ERR5470710.fastq.gz	Fastqsanger.gz
	ERR5470720.fastq.gz	Fastqsanger.gz

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Project description:Lifestyle intervention can improve insulin sensitivity in obese youth yet few studies have examined the biological mechanisms underlying improvements. Therefore, the purpose of this study was to explore biological pathways associated with intervention-induced improvements in insulin sensitivity. Fifteen (7M/8F) overweight/obese (BMI percentile=96.3M-BM-11.1) Latino adolescents (15.0M-BM-10.9 years) completed a 12-week lifestyle intervention that included weekly nutrition education and 180 minutes of moderate-vigorous exercise per week. Insulin sensitivity, estimated by an oral glucose tolerance test and the Matsuda Index, increased 29.2% post intervention (2.4M-BM-10.3 to 3.1M-BM-10.3, p=0.01). Global microarray analysis profiling from whole blood was performed to examine changes in gene expression and to explore biological pathways that were significantly changed in response to the intervention. A total of 1,459 probes corresponding to mRNA transcripts (717 up, 742 down) were differentially expressed with a fold changeM-bM-^IM-%1.2 and P<0.05. Among the genes identified were hexokinase 3 (HK3), ATPase, H+ transporting V0 subunit e2 (ATPV0E), and sterol regulatory element binding transcription factor 1 (SREBF1), and endothelial cell adhesion molecule (ESAM). There were 8 pathways identified that met the criteria for significance, including insulin signaling, type 1 diabetes, and glycerophospholipid metabolism. Participants that increased insulin sensitivity exhibited five times the number of significant genes altered compared to non-responders (1,144 vs. 230). These findings offer insight into the molecular mechanisms underlying health improvements among high-risk Latino youth. Lifestyle interventions may contribute to improved insulin sensitivity through pathways related to insulin signaling and immune response. Further, genetic factors may mediate response to lifestyle intervention. Fifteen (7M/8F) overweight/obese Latino Youth Whole blood RNA samples evaluated pre and post intervention.

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Gut microbiota predicts body fat change following a Low Energy Diet: a PREVIEW sub-study

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