SRPDB
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REPOSITORIES: ENA
Similar Datasets
Project description:5’ ExoSeq of total RNA (rRNA & signal recognition particle RNA depleted) from mouse cortical neurons before and after membrane depolarization by potassium chloride (KCl).
2022-03-08 | GSE163111 | GEO
Project description:Implications of the SRP72 p.R124S mutation on signal recognition particle functions [K562_SRP72]
| PRJNA923605 | ENA
Project description:We generated Bscl2lox/lox mice X ERT2-Adipoq-CRE mice, allowing inducible seipin deletion in adipocytes upon tamoxifen addition (iATSKO). Indeed, a 5-day tamoxifen treatment led to a dramatic decrease in Bscl2 mRNA in the adipose tissue. To study the effects of seipin deficiency in vivo on adipose tissue homeostasis in an unbiased manner, we used 3’-signal recognition particle (SRP) RNA sequencing.
2022-01-11 | GSE188544 | GEO
Project description:Mitochondria from subcutaneous white adipose tissues of control or signal recognition particle 54c (SPR54c) transgenic mice were isolated and underwent further Percoll gradient-based purification. Purified mitochondria were subject to mass-spectrometry based proteomic analysis performed by UT Southwestern Proteomics Core. ID# 696496: SRP54c Transgenic; ID# 696497: control.
2019-10-24 | MSV000084498 | MassIVE
Project description:To test whether non-coding RNAs play roles in regulating response to powdery mildew infection and heat stress in wheat, by using Solexa high-throughput sequencing and computational analysis and experimental approach we cloned the small RNAs and identified 125 putative long npcRNAs from wheat leaves infected by preponderant physiological strain Erysiphe graminis f. sp. tritici (Egt) or by heat stress treatment. Among long non-coding RNAs, some were precursors of small RNAs such as microRNAs and siRNAs, two long npcRNAs were identified as signal recognition particle (SRP) 7S RNA variants, and three were characterized as U3 snoRNAs. Wheat long npcRNAs showed tissue dependent expression patterns and were responsive to powdery mildew infection and heat stress.
2011-02-15 | GSE27327 | GEO
Project description:The signal recognition particle (SRP) enables cotranslational delivery of proteins for translocation into the endoplasmic reticulum (ER), but its full in vivo role remains incompletely explored. We combined rapid auxin-induced SRP degradation with proximity-specific ribosome profiling to define SRP’s in vivo function in yeast. Despite the classic view that SRP recognizes amino-terminal signal sequences, we show that SRP was generally essential for targeting transmembrane domains regardless of their position relative to the amino-terminus. By contrast, many proteins containing cleavable amino-terminal signal peptides were efficiently cotranslationally targeted in SRP’s absence. We also reveal an unanticipated consequence of SRP loss: Transcripts normally targeted to the ER were mistargeted to mitochondria, leading to mitochondrial defects. These results elucidate SRP’s essential roles in maintaining the efficiency and specificity of protein targeting.
2018-01-18 | GSE106329 | GEO
Project description:The Signal Recognition Particle (SRP) is a universally conserved and abundant ribonucleoprotein particle (RNP) in cells. Its composition and size vary across evolution. In mammals, SRP comprises one RNA molecule, the 7SL RNA, and six proteins: SRP19, SRP19, SRP19, SRP54, SRP68, and SRP72. SRP is essential for targeting transmembrane and secreted proteins to the endoplasmic reticulum. To reveal novel aspects of SRP biogenesis, we established the interactome of the GFP-SRP72 protein. To do so, we have produced stable human U2OS cell line expressing GFP-SRP19 and performed stable isotope labeling by amino acids in cell culture (SILAC) proteomic experiment using GFP-SRP19 as bait.
2024-06-07 | PXD042195 | Pride
Project description:The Signal Recognition Particle (SRP) is a universally conserved and abundant ribonucleoprotein particle (RNP) in cells. Its composition and size vary across evolution. In mammals, SRP comprises one RNA molecule, the 7SL RNA, and six proteins: SRP9, SRP14, SRP19, SRP54, SRP68, and SRP72. SRP is essential for targeting transmembrane and secreted proteins to the endoplasmic reticulum. To reveal novel aspects of SRP biogenesis, we established the interactome of the GFP-SRP72 protein. To do so, we have produced stable human U2OS cell line expressing GFP-SRP72 and performed stable isotope labeling by amino acids in cell culture (SILAC) proteomic experiment using GFP-SRP72 as bait.
2024-06-07 | PXD042191 | Pride
Project description:The Signal Recognition Particle (SRP) is a universally conserved and abundant ribonucleoprotein particle (RNP) in cells. Its composition and size vary across evolution. In mammals, SRP comprises one RNA molecule, the 7SL RNA, and six proteins: SRP9, SRP14, SRP19, SRP54, SRP68, and SRP72. SRP is essential for targeting transmembrane and secreted proteins to the endoplasmic reticulum. To reveal novel aspects of SRP biogenesis, we established the interactome of the GFP-SRP72 protein. To do so, we have produced stable human U2OS cell line expressing GFP-SRP9 and performed stable isotope labeling by amino acids in cell culture (SILAC) proteomic experiment using GFP-SRP9 as bait.
2024-06-07 | PXD042192 | Pride
Project description:The Signal Recognition Particle (SRP) is a universally conserved and abundant ribonucleoprotein particle (RNP) in cells. Its composition and size vary across evolution. In mammals, SRP comprises one RNA molecule, the 7SL RNA, and six proteins: SRP14, SRP14, SRP19, SRP54, SRP68, and SRP72. SRP is essential for targeting transmembrane and secreted proteins to the endoplasmic reticulum. To reveal novel aspects of SRP biogenesis, we established the interactome of the GFP-SRP72 protein. To do so, we have produced stable human U2OS cell line expressing GFP-SRP14 and performed stable isotope labeling by amino acids in cell culture (SILAC) proteomic experiment using GFP-SRP14 as bait.
2024-06-07 | PXD051488 | Pride