Project description:Whole genome re-sequencing in tomato reveals variation associated with introgression and breeding events. Here we describe the whole-genome resequencing of eight tomato samples chosen to represent a large range of intra-specific variability and the identification and annotation of novel polymorphisms.Comparison of the sequences with the reference genome yielded more than 4 million single nucleotide polymorphisms (SNPs) and almost 128,000 InDels were detected.This study represents the first whole genome resequencing experiment in cultivated tomato.
Project description:Generalist arthropod herbivores rapidly adapt to a broad range of host plants. However, the extent of transcriptional reprogramming in the herbivore and its hosts associated with adaptation remains poorly understood. Using the spider mite Tetranychus urticae and tomato as models with available genomic resources, we investigated the reciprocal genome-wide transcriptional changes in both spider mite and tomato as a consequence of mite’s adaptation to tomato We used microarray to assess global gene expression in Solanum lycopersicum cv. Moneymaker upon Tetranychus urticae attack by tomato-adapted and non-adapted spider mite lines.
Project description:MSH1 depletion caused whole genome wide Methylome changes in Arabidopsis, to conduct comparative genomic analysis, here we generate methylome for MSH1-RNAi line and transgene segregated out msh1 memory line in tomato
