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Whole-genome sequencing of the CRISPR/Cas9-generated cell lines G12LS and C07 carrying a heterozygous and homozygous mutation in the RB1 gene and their parental human embryonic stem cell line H9.

ABSTRACT: Whole-genome sequencing of the CRISPR/Cas9-generated cell lines G12LS and C07 carrying a heterozygous and homozygous mutation in the RB1 gene and their parental human embryonic stem cell line H9.

PROVIDER: PRJEB44115 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR5654590_1.fastq.gz	Fastqsanger.gz
	ERR5654590_2.fastq.gz	Fastqsanger.gz
	ERR5654591_1.fastq.gz	Fastqsanger.gz
	ERR5654591_2.fastq.gz	Fastqsanger.gz
	ERR5654582_1.fastq.gz	Fastqsanger.gz

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Whole-genome sequencing of the CRISPR/Cas9-generated cell lines G12LS and C07 carrying a heterozygous and homozygous mutation in the RB1 gene and their parental human embryonic stem cell line H9.

Project description:Heterozygous and homozygous mutations were introduced to the human embryonic stem cell line H9 by using the CRISPR/Cas9-system. Since off-target effects can occur and high numbers of SNVs can be acquired during clonal selection, the generated cell lines and the parental cell line were analyzed by whole-genome sequencing.

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