Project description:The mechanisms underlying exercise-induced effects in the skeletal muscle during cancer cachexia progression have not been fully described. Here, we tested the hypothesis that different exercise training protocols could attenuate metabolic impairment in a severe model of cancer cachexia. Moderate-intensity training (MIT) and high-intensity interval training (HIIT) improved running capacity and prolonged lifespan in tumor-bearing rats. HIIT also reduced oxidative stress and reestablished muscle contractile function. An unbiased proteomics screening revealed that COP9 signalosome complex subunit 2 (COPS2), also known as thyroid receptor interacting protein 15 (TRIP15) or ALIEN, is one of the most downregulated proteins at the early stage of cancer cachexia progression. HIIT restored COPS2/TRIP15/ALIEN protein expression to the control levels. Moreover, lung cancer patients with low endurance capacity had lower muscle COPS2/TRIP15/ALIEN protein content compared to age- and sex-matched control subjects. We further established an in vitro model of cancer-induced muscle wasting using tumor cells-conditioned media to explore the potential protective role of COPS2/TRIP15/ALIEN for myotubes homeostasis. This in vitro model indicate that tumor cells produce factors that directly affect myotube metabolism, but COPS2/TRIP15/ALIEN overexpression is not able to fully reestablish metabolic homeostasis and protein content in myotubes incubated with tumor cells-conditioned media. The current study provides new insight into the role of exercise training as a co-therapy for cancer cachexia and uncovers COPS2/TRIP15/ALIEN as a novel potential target for cancer cachexia.
Project description:We report the expression profiles of putative genes involved in temperature-dependent sex determination across multiple developmental stages in turtles, and contrast this data with equivalent stages in turtles with sex chromosomes
Project description:Human activities and climate change have negatively affected the world's oceans, leading to a 30-60% decline in biodiversity and habitats in coastal ecosystems. Marine turtles, as bioindicator species, accumulate contaminants, including trace elements, due to their extensive migration and long life span. However, there is a lack of data on the abundance of these contaminants and their effects on marine turtles' health. This study focuses on analyzing the muscle proteome of juvenile green sea turtles (Chelonia mydas) from Reunion Island. The ultimate goal was to evaluate whether muscle proteome responds to in-situ mixtures of inorganic contaminants to decipher the possible impacts on individual health, thereby identifying potential new biomarkers for long-term monitoring and conservation efforts.
Project description:In the present study, the eggs of Chinese pond turtles (Mauremys reevesii) were incubated at three temperatures (26℃, 29℃ and 32℃). During the thermosensitive period (TSP) of incubation, the adrenal-kidney-gonad (AKG) complexes were sampled, and a comprehensive investigation for miRNAs was performed using next-generation small RNA sequencing.
Project description:In 2010, the Deepwater Horizon oil spill resulted in an estimated loss of 61,000–174,000 sea turtles in the Gulf of Mexico. Rescued Kemp’s ridley sea turtle exhibited adverse effects, including physiological stress and severe derangements. While prior studies evaluated health impacts of oil exposure, effects on plasma proteins remained largely unexplored. This study compared the plasma proteome of turtles from two oiling categories (OC) before and after rehabilitation. Plasma samples were collected from lightly oiled (n=9) and heavily oiled (n=15) turtles at admission and post-rehabilitation. Proteins were analyzed using label-free tandem mass spectrometry. At admission, few differences were observed between groups, with 17 proteins reduced in heavily oiled turtles relative to lightly oiled turtles. After rehabilitation, no significant differences were detected, suggesting normalization of the plasma proteome. Osteocalcin, a bone mineralization and metabolic hormone, displayed a large increase after recovery implicating a mechanistic role for this hormone in the recovery of sea turtles. Unexpectedly, alpha-fetoprotein was the most abundant plasma protein in 41 of 48 samples representing 20.2 ± 3.1% of the molar protein fraction surpassing albumin which is routinely measured for diagnostic evaluation. These findings demonstrate that plasma proteomics can distinguish oiling status and support future biomarker development.