Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genetic Analysis of Plasmid-Encoded mcr-1 Resistance in Enterobacteriaceae derived from poultry meat.

ABSTRACT: Genetic Analysis of Plasmid-Encoded mcr-1 Resistance in Enterobacteriaceae derived from poultry meat.

PROVIDER: PRJEB44175 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR5726840.fastq.gz	Fastqsanger.gz
	ERR5726850.fastq.gz	Fastqsanger.gz
	ERR5726860.fastq.gz	Fastqsanger.gz
	ERR5726870.fastq.gz	Fastqsanger.gz
	ERR5727770_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 105

Similar Datasets

Genetic analysis of plasmid-encoded <i>mcr-1</i> resistance in Enterobacteriaceae derived from poultry meat in the Netherlands.

Project description:BackgroundColistin is classified as the highest priority and critically important antimicrobial for human medicine by WHO as it is the last resort agent for treatment of carbapenem-resistant Enterobacteriaceae in humans. Additional research is necessary to elucidate the genetic structure of mcr-1 resistance genes, commonly found on plasmids, using WGS.ObjectivesTo map and compare the genetic characteristics of 35 mcr-1-mediated colistin-resistant Enterobacteriaceae isolated from chicken meat to highlight the genetic variation of the mcr-1-containing plasmids.MethodsSequencing was performed using Illumina HiSeq2500, Novaseq6000 and ONT's GridION. GridION data was locally basecalled and demultiplexed using ONT's Albacore 2.3.4 followed by Porechop 2.3. Quality filtering was performed using Filtlong 2.0. Hybrid Assembly was performed using Unicycler 4.7. Plasmids were compared with reference sequences in plasmid-RefSeq and pATLAS.ResultsA total of 35 mcr-1 positive Enterobacteriaceae were investigated, which resulted in 34 qualitatively robust hybrid assemblies of 2 Klebsiella pneumoniae and 32 Escherichia coli. mcr-1.1 was present in 33/34 isolates. One isolate contained an mcr-1.1-like resistance gene, due to a deletion of one codon. Two mcr-1.1 genes were located on the chromosome, while the majority of the mcr-1 genes were found on IncX4 type plasmids (n = 19). Almost all plasmids identified in this study were highly similar to plasmids found in human-derived strains.ConclusionsThe mcr-1.1-containing plasmids from retail chicken show high sequence similarity to human mcr-1.1 plasmids, suggesting that this may be a contributor to the presence of colistin resistance in humans.

| S-EPMC8597959 | biostudies-literature

mcr-1-carrying Enterobacteriaceae from chicken meat

Project description:Occurrence of mcr-1-carrying Enterobacteriaceae from chicken meat supplied from Portuguese farms

| PRJEB44904 | ENA

Spiked poultry meat

Project description:Spiked poultry meat

| PRJEB30886 | ENA

Enterobacteriaceae

Project description:ESBL-producing Enterobacteriaceae from poultry

| PRJNA855630 | ENA

IncHI2 mcr-1 Plasmid

Project description:mcr-1 outbreak on swiss Pigfarm

| PRJEB45636 | ENA

Enterobacteriaceae

Project description:Whole genome sequencing of mcr-1-positive Enterobacteriaceae

| PRJDB7066 | ENA

Enterobacteriaceae

Project description:Enterobacteriaceae harboring mcr genes Genome sequencing and assembly

| PRJNA761490 | ENA

Multi-species poultry meat products

Project description:Multi-species poultry meat products

| PRJNA891707 | ENA

Gene expression microarray data by Moutan Cortex Radicis (MCR) in the LPS-stimulated human gingival fibroblast

Project description:The Moutan Cortex Radicis (MCR) has been used as an analgesic, sedative and anti-inflammatory agent. This study investigated the changes in gene expression by MCR treatment when stimulated with lipopolysaccharide (LPS) in cultured human gingival fibroblasts (HGFs) and the gene expression changes by the MCR when challenged with LPS using a microarray chip.

2012-06-28 | GSE38977 | GEO

Enterobacteriaceae

Project description:Identification of mobile colistin resistance genes (mcr-1.1, mcr-5, mcr-8.1) in Enterobacteriaceae of human and animal origins, Nigeria.

| PRJNA631965 | ENA

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data