Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Antagonistic activity of epiphytic Pseudomonas strains against phytopathogenic Pseudomonas syringae pv. syringae

ABSTRACT: Evaluation of antagonistic activity of epiphytic Pseudomonas strains against phytopathogenic Pseudomonas syringae pv. syringae causing bacterial Citrus blast and black pit

PROVIDER: PRJEB44322 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Blue light perception by epiphytic Pseudomonas syringae drives chemoreceptor expression enabling efficient plant infection

Project description:Blue light perception by epiphytic Pseudomonas syringae drives chemoreceptor expression enabling efficient plant infection

2020-10-31 | GSE152188 | GEO

PROMISING EPIPHYTIC ANTAGONISTIC PSEUDOMONAS STRAINS FROM THE CITRUS PHYLLOSPHERE IN THE BIOCONTROL OF PSEUDOMONAS SYRINGAE

Project description:PROMISING EPIPHYTIC ANTAGONISTIC PSEUDOMONAS STRAINS IN THE BIOCONTROL OF PSEUDOMONAS SYRINGAE

| PRJEB51009 | ENA

Pseudomonas syringae Virulence Regulatory Network construction

Project description:Pseudomonas syringae, a Gram-negative plant pathogen, infects more than 50 crops with its type III secretion system (T3SS) and causes severe economic losses around the world. Although the mechanisms of virulence-associated regulators of P. syringae T3SS have been studied for decades, the crosstalk and network underlying these regulators are still elusive. Previously, we have individually studied a group of T3SS regulators, including AefR, HrpS, and RhpRS. In the present study, we found 4 new T3SS regulator genes (envZ, ompR, tsiS and phoQ) via transposon-mediated mutagenesis. Two-component systems EnvZ and TsiS natively regulate T3SS. In order to uncover the crosstalk between 16 virulence-associated regulators, (including AefR, AlgU, CvsR, GacA, HrpL, HrpR, HrpS, MgrA, OmpR, PhoP, PilR, PsrA, RhpR, RpoN, TsiR and Vfr) in P. syringae, we mapped an intricate network named PSVnet (Pseudomonas syringae Virulence Regulatory Network) by combining differentially expression genes in RNA-seq and binding loci in ChIP-seq of all regulators.

2021-03-29 | GSE138204 | GEO

Transcriptional profile of Pseudomonas syringae pv phaseolicola at low temperature

2012-12-31 | GSE38423 | GEO

Diversity of HrpL-regulons across Pseudomonas syringae isolates

Project description:We implemented transcriptional analysis methods using cDNA and high-throughput sequencing data to identify HrpL-regulated genes for six strains of Pseudomonas syringae

2014-01-01 | GSE46930 | GEO

Transcriptional profile of Pseudomonas syringae pv phaseolicola at low temperature

Project description:In order to obtain a global view about the strategies used by phytopathogenic bacteria, in response to physiologically relevant temperature changes. We used the DNA microarray technology to compare gene expression profile in the model bacterial pathogen P. syringae pv. phaseolicola NPS3121 grown at 18 M-BM-:C and 28M-BM-:C. To carry out this study, we used this DNA microarray of P. syringae pv. phaseolicola NPS3121. Each microarray experiment was repeated six times; two technical replicates with the same RNA samples and three biological replicates using RNA isolated from a different culture.

2012-12-31 | E-GEOD-38423 | biostudies-arrayexpress

Diversity of HrpL-regulons across Pseudomonas syringae isolates

Project description:We implemented transcriptional analysis methods using cDNA and high-throughput sequencing data to identify HrpL-regulated genes for six strains of Pseudomonas syringae Each Pseudomonas syringae strains was transformed with either pBAD::EV or pBAD containing native hrpL sequence. Strains were grown in MM media supplemented with arabinose and collected 1, 3, and 5 hours post arabinose treatment. RNA was extracted for each time point and mixed at a 1/3 ratio. After removal of rRNA, double stranded cDNA was generated and library prepared accordeing to Illumina protocols.

2014-01-01 | E-GEOD-46930 | biostudies-arrayexpress

Transcriptome profiling of Pseudomonas syringae pv. syringae 9644 in rich media and hrp-inducing minimum media

Project description:Pseudomonas syringae pv. syringae 9644 (Pss9644) is a causal agent of bacterial cherry canker causing necrotic symptoms on leaves, fruits, gummosis and canker in woody tissues of sweet cherry (Prunus avium). To understand which virulent factor genes were expressed in vitro, Pss9644 was grown in rich media (King's B Broth) and minimum media (hrp-inducing minimum media). The latter mimics the in planta environment.

2024-04-07 | GSE255102 | GEO

Contact-dependent traits in Pseudomonas syringae B728a.

Project description:Many bacteria can transition from a planktonic lifestyle to life attached to a surface. Changes in gene expression have been documented in bacteria in mature biofilms, but few studies have looked at gene expression during the initial stages of surface attachment. To investigate this, we performed RNA-Seq using the model organism Pseudomonas syringae B728a which has been found in rivers and lakes but is known for living on the leaf surface. We compared gene expression of wild-type P. syringae B728a cells attached to a filter for 2 hours to the gene expression of wild-type P. syringae B728a cells in King's medium B broth. We found that certain gene catergories were quickly induced when cells were on a surface such as flagellar synthesis and motility while other gene categories were quickly repressed such as phytotoxin synthesis and transport. These fast changes in gene expression suggest that P. syringae B728a uses surface attachment as a potential cue to better adapt to life on a surface.

2020-09-14 | GSE157877 | GEO

Transcription profiling of Arabidopsis infected with the bacterium Pseudomonas syringae

Project description:The effect of bacterial infection with Pseudomonas syringae on gene expression of Arabidopsis thaliana

2005-01-01 | E-TIGR-3 | biostudies-arrayexpress