RNA-Seq of human fibroblasts exposed to X-ray radiation
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB44913 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| ERR5917780.fastq.gz | Fastqsanger.gz | |||
| ERR5917772.fastq.gz | Fastqsanger.gz | |||
| ERR5917773.fastq.gz | Fastqsanger.gz | |||
| ERR5917774.fastq.gz | Fastqsanger.gz | |||
| ERR5917775.fastq.gz | Fastqsanger.gz |
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Project description:Radioadaptive response (RAR) in mammalian cells refers to the phenomenon where a low-dose ionizing irradiation alters the gene expression profiles, and protects the cells from the detrimental effects of a subsequent high dose exposure. We focused on mRNA microarray studies, and aimed to characterize the transcriptome for RAR in AG 1522 human skin fibroblasts and to examine the functional regulatory networks at the genetic level. AG 1522 cells were exposed at a specific time point to a challenging dose of 2 Gy in the RAR group, or a priming dose of 5 cGy in the low-dose group. We aimed to have a comprehensive investigation on the RAR induced in the AG 1522 human fibroblasts first exposed to 5 cGy (priming dose) and then followed by 2 Gy (challenge dose) of X-ray through comparisons to those cells which had only received a single 2 Gy dose.
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Project description:Radioadaptive response (RAR) in mammalian cells refers to the phenomenon where a low-dose ionizing irradiation alters the gene expression profiles, and protects the cells from the detrimental effects of a subsequent high dose exposure. We focused on microRNA microarray studies, and aimed to characterize the transcriptome for RAR in AG 1522 human skin fibroblasts and to examine the functional regulatory networks at the genetic level. AG 1522 cells were exposed at a specific time point to a challenging dose of 2 Gy in the RAR group, or a priming dose of 5 cGy in the low-dose group. We aimed to have a comprehensive investigation on the RAR induced in the AG 1522 human fibroblasts first exposed to 5 cGy (priming dose) and then followed by 2 Gy (challenge dose) of X-ray through comparisons to those cells which had only received a single 2 Gy dose.
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Project description:Background: In the event of an improvised nuclear device or radiological dirty bomb detonation large numbers of people will be exposed to radiation that would require a timely and accurate biodosimetry to identify the highly exposed individuals who would require medical treatment from those who have received low or no radiation (the “worried well”). Gene expression signatures in response to radiation have been derived from mice and human peripheral blood and the impact of a number of variables, such as dose, time, genotype, and gender, on gene expression has been analyzed. However, the impact of aging on radiation gene profiling has not been taken into consideration. Results: Global gene expression was measured in the blood of young (2 mo) and old (24 mo) female C57BL/6J mice 1 day after they were exposed to 4 Gy x-rays or they were sham irradiated (control) using the Agilent Mouse Whole Genome microarrays. Ingenuity Pathway Analysis revealed that “Integrin signaling” was the most overrepresented canonical pathway in both young (p = 1.69E-10) and old (p = 2.93E-10) female mice exposed to x-ray irradiation. In contrast, immune-related processes, such as “Communication between Innate and Adaptive Immune Cells” (p = 2.33E-11), were overrepresented in young but not old irradiated mice. On the other hand, “Molecular Mechanisms of Cancer” processes were significant only in irradiated old mice (p = 5.78E-07). Finally, a number of phagocytosis pathways, involved in apoptotic cell elimination and tissue homeostasis preservation, were enriched in irradiated young, but not old, mice. Conclusions: We show that age is a variable that has the potential to fundamentally alter the transcriptomic profile of irradiated female mouse blood. A number of biological processes, including phagocytosis, are differentially represented in young and old mice exposed to x-ray radiation. Our results highlight the significance of age as a variable that can have profound biological effects that will affect medical management and treatment decisions in case of a radiological emergency.
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