RNA-seq of hiPSCs-derived hepatocytes cultured in 3D and treated with oleic or palmitic acid
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB45771 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| ERR6087140_1.fastq.gz | Fastqsanger.gz | |||
| ERR6087140_2.fastq.gz | Fastqsanger.gz | |||
| ERR6087141_1.fastq.gz | Fastqsanger.gz | |||
| ERR6087141_2.fastq.gz | Fastqsanger.gz | |||
| ERR6087142_1.fastq.gz | Fastqsanger.gz |
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Project description:A1AT deficiency is an autosomal not recessive disorder caused by mutations in the SERPINA1 gene. Individuals with the Z variant retain polymerised protein in the endoplasmic reticulum of hepatocytes, predisposing them to liver disease. This study primarily aimed to uncover the molecular mechanisms that link protein misfolding to liver injury. To that end, RNA was extracted from hepatocytes differentiated from hIPSCs carrying the Z variant and mutation-corrected hIPSCs (control). The second objective of the study was to benchmark the gene expression profile of both hIPSC-derived hepatocytes types to primary hepatocytes of wild type and a Z variant A1AT genotype.
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