Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Paramecium macronuclear epigenome

ABSTRACT: ChIPseq and MNAse of the Paramecium tetraurelia macronuclear chromatin

PROVIDER: PRJEB46233 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	ERR6281640_1.fastq.gz	Fastqsanger.gz
	ERR6281640_2.fastq.gz	Fastqsanger.gz
	ERR6281910.fastq.gz	Fastqsanger.gz
	ERR6281641_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 34

Similar Datasets

DNA 5-methylcytosine in Paramecium

Project description:5-methyl-cytosine DNA methylation regulates gene expression and developmental programming in a broad range of eukaryotes. However, its presence and potential roles in ciliates, complex single-celled eukaryotes with germline-somatic genome specialization via nuclear dimorphism, are largely uncharted. While canonical cytosine methyltransferases have not been discovered in published ciliate genomes, recent studies performed in the stichotrichous ciliate Oxytricha trifallax suggest de novo cytosine methylation during macronuclear development. In this study, we applied bisfulfite genome sequencing, DNA mass spectrometry and antibody-based fluorescence detection to investigate the presence of DNA methylation in Paramecium tetraurelia. While the antibody-based methods suggest cytosine methylation, DNA mass spectrometry and bisulfite sequencing reveal that levels are actually below the limit of detection. Our results suggest that Paramecium does not utilize 5-methyl-cytosine DNA methylation as an integral part of its epigenetic arsenal.

2018-04-01 | GSE111621 | GEO

Structural maintenance of chromosomes (SMCs) proteins are required for developmental DNA elimination in Paramecium tetraurelia

Project description:To study the role of structural maintenance of chromosome (SMC)4-1 and SMC4-2 during programmed genome rearrangement in Paramecium tetraurelia. Paramecium SMC4-1 and SMC4-2 was tagged with 3 FLAG and HA at its C-terminal separately. The recombinant plasmid was microinjected into macronuclear and used for co-immunoprecipitation and Mass spectrometry studies to identify interacting proteins of SMC4-1 and SMC4-2 that indicates the different functions between Paramecium SMC4s.

2023-12-05 | PXD041826 | Pride

Transcriptome analysis, Paramecium tetraurelia ICL (as a control) vs. Rdr3 silencing

Project description:Differential transcriptome of Paramecium tetraurelia strain 51 undergoing RNAi by feeding against ICL7a (as a control) and RDR3 for nine days.

2014-07-15 | GSE59390 | GEO

Paramecium tetraurelia strain d4-2

Project description:The macronuclear genome of Paramecium tetraurelia has been sequenced by the Paramecium Genomics Consortium

| PRJNA12726 | ENA

ASI

Project description:Paramecium tetraurelia macronuclear strain 51

| PRJEB3076 | ENA

Paramecium tetraurelia

Project description:Paramecium tetraurelia reciliation (log phase)

| PRJNA154017 | ENA

Paramecium tetraurelia

Project description:Paramecium tetraurelia reciliation (stationary phase)