Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq of Hml-SwitchGal4 induced pathway activation in Drosophila plasmatocytes

ABSTRACT: RNA-seq of Hml-SwitchGal4 induced pathway activation in Drosophila plasmatocytes

PROVIDER: PRJEB46481 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR6336630_1.fastq.gz	Fastqsanger.gz
	ERR6336630_2.fastq.gz	Fastqsanger.gz
	ERR6336640_1.fastq.gz	Fastqsanger.gz
	ERR6336640_2.fastq.gz	Fastqsanger.gz
	ERR6336650_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 72

Similar Datasets

RNA-seq of Hml-SwitchGal4 induced pathway activation in Drosophila plasmatocytes

Project description:Drosophila carrying Hml-SwitchGal4 were crossed to different uas-constructs (uas-eGFP, uas-PGRP-LC, uas-hop, uas-tkvQD). 3rd instar larvae were either kept as control or SwitchGal4 was activated by hormone feeding for 24h. Afterwards plasmatocytes were extracted and subjected to RNA-seq.

2022-05-31 | E-MTAB-10764 | biostudies-arrayexpress

RNA-seq of plasmatocytes after septic injury with trxG-RNAis

Project description:Drosophila plasmatocyes expressed control or trxG RNAis under the plasmatocyte specific Hml promotor. 6h prior to plasmatocyte collection animals were split into control and septic injury groups and treated accordingly. Afterwards plasmatocytes were isolated and polyA RNA was subjected to RNA-seq.

2022-05-31 | E-MTAB-10780 | biostudies-arrayexpress

Activation of HERV-K(HML-2) limits cortical neuronal differentiation by modulating Neurotrophic Tyrosine Receptor Kinase 3 expression

Project description:The biological function and disease association of human endogenous retroviral (HERV) elements remains largely elusive. We addressed the physiological role of HERV-K(HML-2) in neuronal differentiation by manipulating HERV-K(HML-2) expression levels. We used CRISPR engineering to activate or repress HERV-K(HML-2) and demonstrate that elevated HERV-K(HML-2) transcription is detrimental for development, functionality and growth of cortical neurons. Effects are cell-type specific, as dopaminergic neurons were unaffected. We further show that layer formation is altered during forebrain organoid formation following activation of HERV-K(HML-2) transcription. HERV-K(HML-2) transcriptional activation concurrently elevated Neurotrophic Tyrosine Receptor Kinase 3 (NTRK3) expression along with other neurodegeneration-related genes. Direct transcriptional activation of NTRK3 resembled the HERV-K(HML-2) activation phenotype. Intriguingly, reduction of NTRK3 levels in HERV-K(HML-2)-activated cortical neurons restored differentiated cortical neurons. Hence, our findings unravel a unique cell type-specific mechanism of HERV-K(HML-2) during cortical neuronal differentiation.

2021-03-30 | GSE171101 | GEO

ChIP-seq of Drosophila melanogaster OregonR plasmatocytes at 6h post septic injury

Project description:Drosophila 3rd instar larvae were subjected to septic injury. After 6h plasmatocytes were isolated, fixed and subjected to ChIP-seq.

2022-05-31 | E-MTAB-10760 | biostudies-arrayexpress

ChIP-seq of Drosophila melanogaster plasmatocytes against various histone modifications

Project description:To identify the presence of different chromatin states in homogeneous primary cells, Drosophila melanogaster plasmatocytes were isolated from 3rd instar larvae and subjected to cross-linked ChIP-seq using antibodies against a range of H3 histone modifications and PolII.

2022-05-31 | E-MTAB-10707 | biostudies-arrayexpress

RNA-seq of plasmatocytes after septic injury

Project description:OrR drosophila 3rd instar larvae were subjected to septic injury with a mixture of E.coli and S.aureus at 3h, 6h and 18h. Plasmatocytes were isolated afterwards and subjected to RNA-seq

2022-05-31 | E-MTAB-10761 | biostudies-arrayexpress

ChIP-seq of Drosophila melanogaster plasmatocytes agains various histone modifications

Project description:ChIP-seq of Drosophila melanogaster plasmatocytes agains various histone modifications

| PRJEB46189 | ENA

Mechanisms of HERV-K (HML-2) transcription during human mammary epithelial cell transformation

Project description:Elevated transcript expression of the endogenous retrovirus family HERV-K (HML-2) is seen in the majority of breast cancers, although the identity of the individual loci contributing to this expression as well as their mechanism of activation is unclear. Using high-throughput next-generation sequencing techniques optimized for the capture of HML-2 expression, we produced a complete profile of the HML-2 transcriptome before and after human mammary epithelial cell transformation.

2017-10-13 | GSE84275 | GEO

RNA-seq of resting plasmatocytes from Drosophila melanogaster third instar larvae

Project description:Resting plasmatocytes were isolated from 3rd instar larvae and subjected to RNA-seq as comparison to ChIP-seq data

2022-05-31 | E-MTAB-10759 | biostudies-arrayexpress

RNA-seq of resting plasmatocytes from Drosophila melanogaster third instar larvae

Project description:RNA-seq of resting plasmatocytes from Drosophila melanogaster third instar larvae

| PRJEB46505 | ENA