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RNAseq of pancreatas from KRasG12D/RosaMycER+/- or KRasG12D/RosaMycER+/+ treated with Tamoxifen for 12h

ABSTRACT: RNAseq of pancreatas from KRasG12D/RosaMycER+/- or KRasG12D/RosaMycER+/+ treated with Tamoxifen for 12h

PROVIDER: PRJEB46823 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR6434280.fastq.gz	Fastqsanger.gz
	ERR6434271.fastq.gz	Fastqsanger.gz
	ERR6434281.fastq.gz	Fastqsanger.gz
	ERR6434272.fastq.gz	Fastqsanger.gz
	ERR6434282.fastq.gz	Fastqsanger.gz

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RNAseq of pancreatas from KRasG12D/RosaMycER+/- or KRasG12D/RosaMycER+/+ treated with Tamoxifen for 12h

Project description:Experiment designed to study the effect of 1 or 2 copies of deregulated myc on transcriptional profile during pancreatic cancer progression. In the KRasG12D/RosaMycER mouse model KRas and MycER are expressed exclusively in the pancreas, but MycER activity depends on Tamoxifen presence. RNAseq was preformed on pancreatas from KRasG12D/RosaMycER+/- or KRasG12D/RosaMycER+/+ mice treated 12 hours with Tamoxifen.

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RNAseq of cell line from pancreas from KRasG12D/RosaMycER mouse treated with Tamoxifen

Project description:Experiment designed to study the effect of deregulated myc on pancreatic cancer progression and regression. In the KRasG12D/RosaMycER mouse model KRas and MycER are expressed exclusively in the pancreas, but MycER activity depends on Tamoxifen presence. For this experiment a mouse was treated with Tamoxifen during 3 weeks, the pancreas was collected and the epithelial cells were grown in vitro in the presence of 4-OHT (4-Hydroxytamoxifen, active form of Tamoxifen). RNAseq were preformed on the cell line treated as follows: 1- 4-OHT all the time. 2- EtOH 6h. 3- EtOH 12h. 4- EtOH 18h.

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RNAseq of pancreata from KRasG12D/RosaMycER treated with Tamoxifen for different times

Project description:Experiment designed to study the effect of deregulated myc on pancreatic cancer progression and regression. In the KRasG12D/RosaMycER mouse model KRas and MycER are expressed exclusively in the pancreas, but MycER activity depends on Tamoxifen presence. RNAseq was preformed on pancreata from mice treated with Tamoxifen as follows: 1- Normal food. 2- Tamoxifen food 2 weeks. 3- Tamoxifen food 2 weeks, followed by normal food 1 day. 4- Tamoxifen food 2 weeks, followed by normal food 3 days.

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RNAseq of pancreatas from KRasG12D/RosaMycER treated with Tamoxifen for different times. MycOFF short times.

Project description:Experiment designed to study the effect of deregulated myc on pancreatic cancer progression and regression. In the KRasG12D/RosaMycER mouse model KRas and MycER are expressed exclusively in the pancreas, but MycER activity depends on Tamoxifen presence. RNAseq was preformed on pancreatas from mice treated with Tamoxifen as follows: 1-Tamoxifen food 2 weeks. 2- Tamoxifen food 2 weeks, followed by normal food 6 hours day. 3- Tamoxifen food 2 weeks, followed by normal food 12 hours.

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