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IlEulPrun

ABSTRACT: Eulithis prunata (the phoenix)

PROVIDER: PRJEB46849 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
		Other
	CAKKOA01.dat.gz	Other
	CAKKOA01.fasta.gz	Fasta.gz
	CAKKOA01.master.dat	Other
	ERR6688625_1.fastq.gz	Fastqsanger.gz

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WNT activation and TGFβ-smad inhibition potentiate stemness of mammalian auditory neuroprogenitors for high throughput generation of functional auditory neurons in vitro

Project description:We have previously identified and characterized the phoenix auditory neuroprogenitors (ANPGs) as highly proliferative progenitor cells isolated from the A/J mouse cochlea. In the present study, we aimed at identifying signaling pathways responsible for the intrinsic high stemness of phoenix ANPGs. A transcriptomic comparison of traditionally low stemness ANPGs, isolated from C57Bl/6 and A/J mice at early passages, and high stemness phoenix ANPGs was performed.

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Project description:phoenix (pho) is a naturally occurring rice (Oryza sativa L.) mutant that exhibits stable inflorescence reversion. Genetic analysis showed that it was not a simple mendelian mutant. In order to find the major genes contributed to the formation of pho, microarray experiments were carried out to analyze and compare the transcriptional profiles between pho and wild-type panicles. We generated gene expression profiles of rice Indica cultivar Zhongxian 3037 and mutant phoenix (pho) by using 57K Affymetrix rice whole genome array.

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Project description:Sphere-forming progenitor cells can be isolated from the fetal and adult mammalian inner ear and give rise to inner ear specific cell types in vitro. Here, we provide phenotypical and functional characterization of a new pool of auditory progenitors as sustainable source for sphere-derived auditory neurons. The so-called phoenix auditory neuroprogenitors, isolated from the A/J mouse spiral ganglion, exhibit nearly unlimited intrinsic self-renewal properties (beyond 40 passages). At any passage, phoenix spheres can be efficiently differentiated by withdrawing growth factors into mature spiral ganglion cells, expressing both neurons and glial cells phenotypic markers and exhibiting similar functional properties as mouse spiral ganglion explants and human sphere-derived spiral ganglion cells. The present dataset includes RNAseq-based transcriptome analysis of phoenix auditory neurons following 7 days of differentiation. mRNA levels in differentiated cells are expressed relatively to neuroprogenitor spheres of equivalent passage (paired samples at passage 12, 21 and 36)

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