Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

In Vitro Selection of DNA Molecules Favouring Intramolecular Circularization by DNA Ligase

ABSTRACT: In Vitro Selection of DNA Molecules Favouring Intramolecular Circularization by DNA Ligase

PROVIDER: PRJEB46967 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR6496565_1.fastq.gz	Fastqsanger.gz
	ERR6496565_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 2 of 2

Similar Datasets

RNA Proximity Ligation to Resolve Intramolecular RNA Structures in situ

Project description:Proof-of-concept of a new method involving the limited digestion and subsequent ligation of intramolecular RNA structures in situ followed by deep sequencing Proof-of-concept of RPL in S. cerevisiae and H. sapiens tissue culture

2015-08-01 | E-GEOD-69472 | biostudies-arrayexpress

RNA Proximity Ligation to Resolve Intramolecular RNA Structures in situ

Project description:Proof-of-concept of a new method involving the limited digestion and subsequent ligation of intramolecular RNA structures in situ followed by deep sequencing

2015-08-01 | GSE69472 | GEO

Classifying pathogenic variants in amyloid beta using intramolecular genetic interaction profiling

Project description:Changes in the amino acid sequences of proteins cause thousands of human genetic diseases. However, only a subset of variants in any protein is typically pathogenic, with variants having a diversity of molecular consequences. Determining which of the thousands of possible variants in any protein have similar molecular effects is very challenging, but crucial for identifying pathogenic variants, determining disease mechanisms, understanding clinical phenotypic variation, and developing targeted therapeutics. Here we present a general method to classify variants by their molecular effects that we term intramolecular genetic interaction profiling. The approach relies on the principle that variants with similar molecular consequences have similar genetic interactions with other variants in the same protein. These intramolecular genetic interactions are straightforward to quantify for any protein with a selectable function. We apply intramolecular genetic interaction profiling to amyloid beta, the protein that aggregates in Alzheimer’s disease (AD) and is mutated in familial AD (fAD). Genetic interactions identify two classes of gain-of-function variants, with all known familial Alzheimer’s disease variants having very similar genetic interaction profiles, consistent with a common gain-of-function mechanism leading to pathology. We believe that intramolecular genetic interaction profiling is a powerful approach for classifying variants in disease genes that will empower rare variant association studies and the discovery of disease mechanisms.

2023-11-15 | GSE247583 | GEO

Paedocypris carbunculus isolate:Pooled DNA molecules

Project description:Paedocypris carbunculus isolate:Pooled DNA molecules Raw sequence reads

| PRJNA638469 | ENA

Long cell-free DNA molecules in maternal plasma

Project description:We revealed a large population of long cell-free DNA molecules (up to 23,635 bp in length) in maternal plasma and developed an approach which leveraged the abundance of CpG sites on long molecules to deduce the tissue of origin of individual plasma DNA molecules based on single-molecule methylation analysis. We illustrated how such an approach may be utilized to achieve noninvasive prenatal testing of monogenic diseases. We also revealed a reduction in amounts of such long cell-free DNA molecules and a different end motif profile in maternal plasma DNA from pregnancies with preeclampsia.

| EGAS00001005515 | EGA

ccRNA identification by circularization and miSeq

Project description:ccRNA identification by circularization and miSeq

| PRJNA1158715 | ENA

ccRNA identification by circularization and miSeq

Project description:Efforts to identify ccRNA by sequencing by gibson circularization after template-switching 5' RACE using a cRNA-end specific primer and subsequent sequencing by Illumina miSeq. Sequencing of wild-type A/WSN/1933 and a variant bearing the mutation T677A in the PB1 subunit.

2024-09-10 | GSE276698 | GEO