Project description:Skin homeostasis is guided by spatiotemporal regulation of gene expression, establishing each stage of keratinocyte differentiation. The current study presents transcriptomic and chromatin profiling for the proliferating (basal) and differentiated (suprabasal) cell populations derived from neonate mice (p0-p2) skin epidermis. This multi-omic approach will enable idenitification of cell specific epidermal cross-talk central to the equlibirum.
Project description:Measuring transcriptomic profiles of human embryos cultured in time-lapse imaging (TLI) incubator and standard incubator (SI) is crucial for studying whether the closed culture system has a positive impact on embryos. Here we used RNA-sequencing (RNA-Seq) to characterize and compare the gene expression profiles of TLI and SI cultured 8-cell embryos with the same quality grade. A total of 580,952,620 fragments were sequenced for zygote, SI and TLI groups. The global transcriptomic profiles of embryos from TLI group were similar to SI group, with a high distinct to zygotes. However, wo also detected 539 genes showing differential expression between the TLI and SI group with false discovery rate (FDR) < 0.05. Using GO enrichment analysis, we found that the high expressed genes in SI tend to execute functions, including transcription, RNA splicing and DNA repair, and the high expressed genes in TLI were enriched in the cell differentiation and methyltransferase activity pathway. This study provides the first comparison between SI and TLI via transcriptome analysis, and will serve as a basis for assessing the safety of TLI application in assisted reproductive technology.
Project description:Analysis of genes dysregulated in the lungs of neonates with ACDMPV neonate lungs compared to control lungs Total RNA extracted by eight ACDMPV lungs compared to total RNA extracted from five control lungs
Project description:Fetal growth restriction (FGR) causes a wide variety of developmental defects in the neonate which can lead to heart disease, diabetes, anxiety and other disorders later in life. The effect of FGR on the immune system, however, is poorly understood. Immune cells throughout development are identified using cell-surface markers to distinguish between subtly different stages. These (surface) proteins, however, do not necessarily generate differences in cellular activity and may thus be only a coincidental shallow guide to classifying cells. High-throughput single-cell transcriptomics using DropSeq was performed on mouse neonate thymuses. The T-cell population was selected through flow cytometry in wild type controls and a placental specific isoform of Igf2 knock-down (igf2-p0). Using this analysis, we discovered skewed T-cell populations in the growth restricted murine neonate indicating a developmental delay. This finding recapitulates the altered immunity found in growth restricted human infants. The T-cell deficit persisted into adulthood, even when body and organ weights approached normal levels due to catch-up growth. This reduction in T-cellularity may have significant implications in adult immunity, adding to the wide variety of fetal origins for adult disease already known.
