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RNA-seq of the pituitary gland of male rats exposed to stress against unexposed controls

ABSTRACT: RNA-seq of the pituitary gland of male rats exposed to stress against unexposed controls

PROVIDER: PRJEB49841 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR7912730_1.fastq.gz	Fastqsanger.gz
	ERR7912730_2.fastq.gz	Fastqsanger.gz
	ERR7912740_1.fastq.gz	Fastqsanger.gz
	ERR7912740_2.fastq.gz	Fastqsanger.gz
	ERR7912731_1.fastq.gz	Fastqsanger.gz

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RNA-seq of the pituitary gland of male rats exposed to stress against unexposed controls

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Project description:Environmental stressors such as repeated social defeat may trigger powerful activation of sub-conscious parts of the brain. We examine the consequences of such stress in male rats on the pituitary gland. We prepared 10 Sprague Dawley rats. 5 of them were exposed to stress induced by the resident-intruder paradigm while the other 5 were controls.After dislocation of the neck under isoflurane anaesthesia, the pituitary gland was harvested. To depict an initial epigenetic pituitary stress response, total DNA was isolated from the tissues and used for bisulfite DNA sequencing. This data presented here is a subset of the samples involved in E-MTAB-11285 RNA-seq project.

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Whole-genome bisulfite sequencing of the pituitary gland of male rats exposed to stress against unexposed controls

Project description:Whole-genome bisulfite sequencing of the pituitary gland of male rats exposed to stress against unexposed controls

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Project description:The pituitary gland exhibits sex differences in its function. Diseases associated with dysregulation of the pituitary are also sex-biased in prevalence. Previous qPCR profiling of puberty-related genes in the pituitary gland revealed increasingly sex-biased expression of genes profiled across pubertal transition. Here, we performed small RNA-seq on total RNA extracted from C57BL/6J mouse pituitary gland of both sexes mice at 4 ages spanning pubertal transition (postnatal days 12, 22, 27, 32) (6 replicates per sex at each age) to examine microRNA (miRNA) regulation of sex differences in gene expression observed. Total RNA was sent to SickKids TCAG core to construct small RNA-seq libraries using NEBNext Small RNA Library Prep Kit according to manufacterer’s protocol. Resulting single-end libraries were sequenced at TCAG core on the Illumina HiSeq 2500 v4 flow cell with SR50 bp. Data obtained was processed using miRDeep2 pipeline to align small RNA-seq reads to the mouse genome (mm10) and to filter for miRNAs.

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Expression QTL (eQTL) mapping in the anterior pituitary gland using DES-treated COPxACI F2 rats.

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