Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Whole-genome bisulfite sequencing of the pituitary gland of male rats exposed to stress against unexposed controls

ABSTRACT: Whole-genome bisulfite sequencing of the pituitary gland of male rats exposed to stress against unexposed controls

PROVIDER: PRJEB50010 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR7975600_1.fastq.gz	Fastqsanger.gz
	ERR7975600_2.fastq.gz	Fastqsanger.gz
	ERR7975601_1.fastq.gz	Fastqsanger.gz
	ERR7975601_2.fastq.gz	Fastqsanger.gz
	ERR7975602_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 20

Similar Datasets

Whole-genome bisulfite sequencing of the pituitary gland of male rats exposed to stress against unexposed controls

Project description:Environmental stressors such as repeated social defeat may trigger powerful activation of sub-conscious parts of the brain. We examine the consequences of such stress in male rats on the pituitary gland. We prepared 10 Sprague Dawley rats. 5 of them were exposed to stress induced by the resident-intruder paradigm while the other 5 were controls.After dislocation of the neck under isoflurane anaesthesia, the pituitary gland was harvested. To depict an initial epigenetic pituitary stress response, total DNA was isolated from the tissues and used for bisulfite DNA sequencing. This data presented here is a subset of the samples involved in E-MTAB-11285 RNA-seq project.

2022-08-03 | E-MTAB-11290 | biostudies-arrayexpress

RNA-seq of the pituitary gland of male rats exposed to stress against unexposed controls

Project description:Environmental stressors such as repeated social defeat may trigger powerful activation of sub-conscious parts of the brain. We examine the consequences of such stress in male rats on the pituitary gland. We prepared 20 Sprague Dawley rats. 10 of them were exposed to stress induced by the resident-intruder paradigm while the other 10 were controls. After dislocation of the neck under isoflurane anaesthesia, the pituitary gland was harvested. Total RNA was isolated from the tissues and used in mRNA sequencing.

2022-08-03 | E-MTAB-11285 | biostudies-arrayexpress

RNA-seq of the pituitary gland of male rats exposed to stress against unexposed controls

Project description:RNA-seq of the pituitary gland of male rats exposed to stress against unexposed controls

| PRJEB49841 | ENA

Proteomic analysis of human anterior pituitary gland

Project description:The regulation of pituitary function via the hypothalamus and via intra-pituitary connections represents a complex system. Though hormones secreted from the pituitary glands have been well studied, overall information of proteins expressed in the pituitary glands is very limited. Protein expression profiling of normal pituitary tissue may lead to discovery of novel proteins playing an important role in the physiology of pituitary glands and can lead to better understanding of pituitary gland diseases. We aimed to carry out systematic proteomic profiling of adenohypophysis from human pituitary glands using high-resolution Fourier transform mass spectrometer. A total of 2,175 proteins were identified in this study of which, 105 proteins were identified for the first time as compared to high throughput proteomic-based studies from human pituitary glands. The comprehensive list of proteins identified in this study will facilitate the better understanding the role of this important gland in health and disease.

2019-01-02 | PXD005819 | Pride

Transcription profiling of rat anterior pituitary gland using DES-treated COPxACI F2 rats - Expression QTL mapping.

Project description:Anterior pituitary glands were isolated from 21 week old male rats treated for 12 weeks with the synthetic estrogen diethylstilbestrol (DES). 43 COPxACI F2 animals were selected based on pituitary weight phenotypic extremes: 22 highest and 21 lowest pituitary weights in our F2 population. Expression profiles were determined using Affymetrix Rat Genome 230 v. 2.0 arrays. In conjunction with genomewide genotypes available for these 43 animals, linkage analysis was done using mRNA abundance of each transcript on the array as a separate quantitative phenotype. These arrays provide a resource for expression QTL (eQTL) mapping on a genomewide level in the pituitary gland. Experiment Overall Design: 43 samples from anterior pituitary gland were analyzed. COPxACI F2 male animals were treated with DES for 12 weeks, starting at 9 weeks of age. Pituitary glands were harvested and weighed at sacrifice (21 weeks of age). The 43 samples were selected for pituitary weight phenotypic extremes: 22 highest and 21 lowest pituitary weights in our F2 population. Genomewide genotypes are available for these animals and in conjunction with the array data, genomewide expression QTL (eQTL) mapping was done.

2008-06-12 | E-GEOD-4081 | biostudies-arrayexpress

Transcription profiling of rat anterior pituitary gland response to diethylstilbestrol

Project description:This SuperSeries is composed of the following subset Series:; GSE4028: Effects of diethylstilbestrol (DES) on the anterior pituitary gland of the ACI, Copenhagen and Brown Norway Rat. GSE4080: Effect of DES-treated Ept congenic rat lines on gene expression in the anterior pituitary gland. GSE4081: Expression QTL (eQTL) mapping in the anterior pituitary gland using DES-treated COPxACI F2 rats. Experiment Overall Design: Refer to individual Series

2008-06-12 | E-GEOD-4083 | biostudies-arrayexpress

Exploration of the Chicken Pituitary Gland

Project description:The pituitary gland is a neuroendocrine organ that is involved in several processes within the body such as metabolism, growth, immune function, and reproduction. Increased ambient temperatures are environmental stressor that leads to several welfare concerns in poultry production but also economic losses. Because of the involvement of the pituitary gland in several processes that are affected by heat stress, it is hypothesized this tissue's gene expression will be impacted by heat stress. The objectives of the project are to (a) identify genes that constitue the pituitary gland when compared to other collected chicken tissues (Insert tissues) and (b) identify genes that respond to heat stress via differential expression analysis to better understand the chicken's response to heat at the transcriptomic level.

2017-02-10 | GSE89297 | GEO

Expression QTL (eQTL) mapping in the anterior pituitary gland using DES-treated COPxACI F2 rats.

2006-03-31 | GSE4081 | GEO

Single-Cell Transcriptomics Identifies Pituitary Gland Population Changes in Diet-Induced Obesity

Project description:The obesity incidence is increasing worldwide with the urgent need to identify new therapeutics. Obesity is associated with endocrine alterations, arising from changes in the hypothalamic-pituitary hormone axes, leading to reproductive disorders, altered basal metabolism and stress hormone production. The focus of this study was to determine if diet induced obesity alters pituitary gland plasticity, gene expression and hormone production and secretion. Here we performed single cell RNA-sequencing (scRNA-seq) on pituitary glands from control (CTRL) and high fat diet (HFD) fed males and looked at cell type population dynamics and gene expression changes between HFD and CTRL for all hormone-producing cell types. Our study revealed diet-induced changes in pituitary gland plasticity and hormone production that may play a role in altered hormone production in obese patients and consequently, dysregulation of pituitary gland homeostasis.

2024-01-24 | GSE240022 | GEO

Genome-wide Methylation in Cordblood CD4+ Cells from Newborns Exposed vs. Unexposed to Maternal Tobacco Smoke During Pregnancy

Project description:We generated genome-wide methylation levels, using whole-genome bisulfite sequencing, for sorted CD4+ cells from cordblood of newborns exposed vs. unexposed to tobacco smoke in utero

2019-03-13 | GSE109212 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data