Project description:The aim of this study was to investigate the effect of hsa-miR-363-3p on global gene expression in T-cell acute lymphoblastic leukemia (T-ALL). For this purpose we selected DND-41 T-ALL cell line characterized by high expression of this miRNA. The cells were transduced either with miRZip pGreenPuro scrambled vector (System Biosciences) or miRZip anti-hsa-miR-363-3p vector, each variant in three biological replicates. All 6 libraries were sequenced on Illumina NovaSeq6000 platform, in 2x150 mode, with a coverage of 60M paired reads/sample.
Project description:ATAC-seq on human cell line DND-41 For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf
Project description:Cut&Run analysis was performed in DND-41 cells to analyze the basal level of H3K27ac and DNA bindings of BRG1 and RUNX1 after DMSO or ACBI-1 treatment.
Project description:H3K27ac Hi-ChIP analysis was performed in MOLT-4 and DND-41 cells to analyze active chromatin-chromatin interactions after DMSO or ACBI-1 treatment.
Project description:ATAC-seq analysis was performed in two T-ALL cell lines (DND-41 and MOLT-4) with either DMSO or ACBI-1 treatment at two different time points (3hr and 24hr).
Project description:RNA-seq analysis was performed in two T-ALL cell lines (DND-41 and MOLT-4) to analyze gene expression changes after 24 hours of 100nM ACBI-1 or DMSO treatment.
Project description:RNA-seq analysis was performed in two T-ALL cell lines (DND-41 and MOLT-4) to analyze gene expression changes after 8 hours of 100nM ACBI-1 or DMSO treatment.
Project description:RNA-seq analysis was performed in two T-ALL cell lines (DND-41 and MOLT-4) to analyze gene expression changes after 3 hours of 100nM ACBI-1 or DMSO treatment.
Project description:To identify the relevant targets of the selected miRNAs, we assessed global transcriptome changes by deep-sequencing total neonatal mouse cardiomyocyte RNA after transfection with hsa-miR-590-3p or hsa-miR-199a-3p Four condition experiment; one replicate per condition; mouse neonatal cardiomyocytes transfected with cel-miR-67, hsa-miR-590-3p and hsa-miR-199a-3p; samples collected 72 hours after transfection
Project description:We report the application of transcriptome sequencing for investigating of the hsa-miR-371a-5p and hsa-miR-518a-3p regulated genes. JAR, JEG-3 and BeWo choriocarcinoma cells were transfected with hsa-miR-371a-5p or hsa-miR-518a-3p inhibitors or control inhibitors. Totally, 237, 132 and 277 genes with > 2 folds change and adjusted P < 0.05 were upregulated in JAR, JEG-3 and BeWo cells respectively after hsa-miR-371a-5p knockdown. Meanwhile, 229, 269 and 191 genes were upregulated in JAR, JEG-3 and BeWo cells respectively after hsa-miR-518a-3p knockdown. The top upregulated genes included many oncogenes or oncogenesis associated ones. Enrichment analysis showed hsa-miR-371a-5p and hsa-miR-518a-3p regulated diverse pathways related to tumorigenesis and metastasis. Our results would be helpful for the searching of early molecular biomarkers and therapeutic targets for gestational trophoblastic neoplasia.