Project description:Bacteriophage Immunoprecipitation Sequencing (PhIP-Seq) datasets of three eHHV-6B-positive SLE patients (three technical replicates), six eHHV-6B-negative SLE patients (three technical replicates), and a negative control lacking sera or plasma (12 technical replicates; Illumina NextSeq) and sequencing data of HHV-6 peptide phage library (two technical replicates; Illumina NextSeq).
Project description:Bacteriophage Immunoprecipitation Sequencing (PhIP-Seq) datasets of three eHHV-6B-positive SLE patients (three technical replicates), six eHHV-6B-negative SLE patients (three technical replicates), and a negative control lacking sera or plasma (12 technical replicates; Illumina NextSeq) and sequencing data of HHV-6 peptide phage library (two technical replicates; Illumina NextSeq).
Project description:Transcript Mapping on Affymetrix ENCODE arrays for Total RNA from 4 different samples (2 or 3 technical replicates of each). All of the four same NB4 samples have also been treated with Retanoic Acid (RA) (2 or 3 technical replicates of each). Three of the four same NB4 samples have also been treated with TPA (2 or 3 technical replicates of each). RA treated NB4 cells can be partially differentiated to Neutrophils and TPA treated NB4 cells can be differentiated to Monocytes. Keywords = Transcript Mapping, Human, Affymetrix, Genome Tiling Arrays Keywords: parallel sample
Project description:The experimental design included three biological replicates for each of the three conditions: Sham-Sham, Sham-CLP and burn-CLP. Liver samples were collected from the rats and the total RNA was analyzed on a Affymetrix RAE230A chip. No technical replicates were included in the study Keywords: other
Project description:T. brucei CPSF73 endogenously TAP-tagged at the C-terminal was affinity purified after treating the cells for 15 min with AN7973 at 10x EC50. Three technical replicates were used. 3 technical replicates of untreated cells served as controls. Inducible GFP-TAP with and without treatment served as additional controls
Project description:To determine the acetylome of S. coelicolor, the wild-type S. coelicolor sample was prepared with three biological replicates and two technical replicates and analyzed. To quantify the acetylome and succinylome, three samples (wild-type, ∆SccobB1, and ∆SccobB2) with three biological replicates were prepared and analyzed. The cells were harvested, digested, and then subjected to affinity enrichment before LC-MS/MS analysis.
Project description:Single cell RNA sequencing (scRNA-seq) can be used to characterize variation in gene expression levels at high resolution. However, the sources of experimental noise in scRNA-seq are not yet well understood. We investigated the technical variation associated with sample processing using the single cell Fluidigm C1 platform. To do so, we processed three C1 replicates from three human induced pluripotent stem cell (iPSC) lines. We added unique molecular identifiers (UMIs) to all samples, to account for amplification bias. We found that the major source of variation in the gene expression data was driven by genotype, but we also observed substantial variation between the technical replicates. We observed that the conversion of reads to molecules using the UMIs was impacted by both biological and technical variation, indicating that UMI counts are not an unbiased estimator of gene expression levels. Based on our results, we suggest a framework for effective scRNA-seq studies.