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Faba bean Hedin /2 and Tiffany reference genomes

ABSTRACT: Genome sequencing and assembly of two faba bean genotypes

PROVIDER: PRJEB52541 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	ERR10111237_1.fastq.gz	Fastqsanger.gz
	ERR10111237_2.fastq.gz	Fastqsanger.gz
	ERR10111238_1.fastq.gz	Fastqsanger.gz
	ERR10111238_2.fastq.gz	Fastqsanger.gz

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Comparative physiological, biochemical and proteomic analyses reveal key proteins and crucial regulatory pathways related to drought stress tolerance in faba bean (Vicia faba L.) leaves

Project description:Drought is one of the most crucial environmental stresses limiting faba bean growth and productivity in the Mediterranean region. In order to explore how the faba bean plant responds to drought stress, a physiological and proteomic analysis was performed in leaf tissue. All physiological parameters were affected by drought. The physiological mechanism underlying the response of faba bean leaves to drought was therefore attributed to the alleviation of oxidative stress via the accumulation of organic solutes such as proline and to the synergistic action of the antioxidant enzyme system (CAT, SOD, APX and GPOX). Proteomic analysis identified 2000 proteins from faba bean leaves, of which were 81 differentially expressed. Of those, 45 were upregulated and 36 were downregulated under drought treatment. GO and KEGG enrichments indicated differentially abundant proteins (DAPs) associated with photosynthesis, antioxidants/detoxifying enzymes, molecular chaperones, biosynthesis of amino acids and secondary metabolites, signal transduction, energy and carbohydrate metabolism and metabolic enzymes. The current results provide evidence for a complex synergetic pathway, in which ROS detoxification mechanisms and photoprotection constituted the major aspect of drought tolerance in faba bean leaves. These results offer a foundational basis regarding the molecular mechanism involved in drought resistance within the faba bean species

2024-04-30 | PXD041759 | Pride

Influence of dietary inclusion of a wet processed faba bean protein concentrate on post-smolt Atlantic salmon (Salmo salar)

Project description:In the present study, a faba bean protein isolate (wBPC) with almost ~80 % crude protein produced by a wet process was investigated in feeds for Atlantic salmon in seawater. Four dietary treatments were tested including one treatment with high inclusion of fishmeal (400 g kg-1, named FM) and three treatments with low fishmeal (216 g kg-1) and increasing inclusions of faba bean protein concentrate (0, 70 and 140 g kg-1) substituting soy protein concentrate (236, 125 and 45 g kg-1), named SPC, BPC7 and BPC14 respectively.

2016-04-20 | E-MTAB-4661 | biostudies-arrayexpress

Air-classified faba bean protein concentrate is efficiently utilized as a dietary protein source by post-smolt Atlantic salmon (Salmo salar)

Project description:High-quality sources of protein for the formulation of feeds of carnivorous fish species such as Atlantic salmon are currently being sought. In an earlier screening trial we evaluated for the first time in Atlantic salmon (Salmo salar) the applicability of air-classified faba bean (Vicia faba) protein concentrate (BPC) inclusions in combination with soy protein concentrate (SPC) and fishmeal (FM) using parr as a model. Based on the results in parr in freshwater, the present study tested the hypothesis that BPC can effectively replace SPC as a dietary protein source in post-smolt Atlantic salmon in seawater. Herein we compare three dietary treatments, including BPC0 (no BPC), BPC20 (20% BPC) and BPC40 (40% BPC). Full details on diet formulation are available in the publication.

2015-08-19 | E-MTAB-3835 | biostudies-arrayexpress

SPET genotyping of NORFAB faba bean panel

Project description:Targeted genotyping of 197 faba bean (Vicia faba L.) accessions using Single primer enrichment technology (SPET).

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Increased diversity of beneficial rhizobia enhances faba bean growth

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iTRAQ-Based Proteomics Analysis Revealed Proteins Involved in Drought Response During the Germination Stage in Faba Bean

Project description:iTRAQ-Based Proteomics Analysis Revealed Proteins Involved in Drought Response During the Germination Stage in Faba Bean

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Transcriptome Characterization of developing bean (Phaseolus vulgaris L.) pods from two genotypes with contrasting seed zinc concentrations.

Project description:Dry bean (Phaseolus vulgaris L.) seeds are a rich source of dietary zinc, especially for people consuming plant-based diets. Within P. vulgaris there is at least two-fold variation in seed Zn concentration. Genetic studies have revealed seed Zn differences to be controlled by a single gene in two closely related navy bean genotypes, Albion and Voyager. In this study, these two genotypes were grown under controlled fertilization conditions and the Zn concentration of various plant parts were determined. The two genotypes had similar levels of Zn in their leaves and pods but Voyager had 52% more Zn in its seeds than Albion. RNA was sequence from developing pods of both genotypes. Transcriptome analysis of these genotypes identified 27,198 genes in the developing bean pods, representing 86% of the genes in the P. vulgaris genome (v 1.0 DOE-JGI and USDA-NIFA). Expression was detected in 18,438 genes. A relatively small number of genes (381) were differentially expressed between Albion and Voyager. Differentially expressed genes included three genes potentially involved in Zn transport, including zinc-regulated transporter, iron regulated transporter like (ZIP), zinc-induced facilitator (ZIF) and heavy metal associated (HMA) family genes. In addition 12,118 SNPs were identified between the two genotypes. Of the gene families related to Zn and/or Fe transport, eleven genes were found to contain SNPs between Albion and Voyager.

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The response to substitution of soybean with faba bean protein concentrate on hepatic transcriptome of Atlantic salmon (Salmo salar)

Project description:The production of carnivorous fish such as Atlantic salmon (Salmo salar) is dependent on the availability of high quality protein required as a sustainable substitute for the formulation of the feeds. Plants have arguably the greatest potential, however a major limitation is associated with the presence of anti-nutritional factors. Investigating novel raw materials involves understanding the physiological consequences of the substitution. The primary aim of the present study was to assess the metabolic response of salmon to increasing inclusion of air-classified faba bean protein concentrate (BPC) in feeds as a replacement for soy (SPC). Specifically, we focused on the hepatic transcriptome response to dietary BPC inclusion over a range including commercially relevant levels (e.g. 11-22%) to levels giving impaired performance (45 %). The present investigation provided a profile of the salmon hepatic response to BPC indicating that fish tolerated moderate substitution of dietary SPC with BPC with no evident negative effects on the hepatic physiology of the fish. The analysis of extreme levels of substitution provided insights into physiological mechanisms that are significantly altered providing the basis for further investigation and improvement.

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