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TraDIS-Xpress analysis of gene functions in Escherichia coli

ABSTRACT: TraDIS-Xpress analysis of gene functions in Escherichia coli

PROVIDER: PRJEB53418 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR9830871.fastq.gz	Fastqsanger.gz
	ERR9830872.fastq.gz	Fastqsanger.gz
	ERR9830873.fastq.gz	Fastqsanger.gz
	ERR9830874.fastq.gz	Fastqsanger.gz
	ERR9830875.fastq.gz	Fastqsanger.gz

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TraDIS-Xpress analysis of colistin stress responses in Escherichia coli

Project description:In past, resistance mechanisms have been identified by analysis of resistant isolates or defined mutants. Recently, high-throughput transposon mutagenesis coupled with sequencing (TraDIS-Xpress) is another approach proving useful for elucidating the roles of genes involved in the overall cellular response to a particular stress. In this study, we used TraDIS-Xpress to determine the role played by genes following exposure to colistin stress. Approximately 10^7 cells from the mutant library were inoculated into LB broth at a range of doubling concentrations of colistin ( 0.25 x MIC, 0.5 x MIC, 1 x MIC, 2 X MIC). Experiments were performed with no induction, or with induction using 0.2 or 1 mM of Isopropyl β-D-1-thiogalactopyranoside (IPTG). All experiments were performed in duplicate.

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