Project description:Untargeted metabolomics data obtained from gut samples from adult Atlantic salmon. Sample (about 200 mg) was transferred into a 1.5 ml tube and eluted with 4x wt/vol of ultra-pure water. After homogenization with a Vortex for 1-2 min the sample was centrifuged at 16.000 g for 10 min. The supernatant was transferred into a spinX centrifuge filter, and centrifuged again (15.000 g/4 C/5 min). The filtrate was collected for LC-MS/MS analysis.
Project description:<p>Testing the effect of blue mussel ensilage as a novel cost-efficient protein feed source for Atlantic salmon feed. Fermentation of novel raw materials for fish feed is a new practice with little prior experience of direct relevance to aquaculture and fish farming. In Trial C, alternative processing of the raw blue mussel meat was tested where the protein was ensilaged before use instead of processed into a dry meal. By using this preservation process, a significant part of small peptides and free amino acids is generated. This trial aimed to examine whether the provision of better protein utilisation and a different microbiome in the gut of the fish can lead to a satisfactory utilisation of this ensilaged protein source by salmon. Earlier published results have shown that ensilaged fish protein provides better protein utilization and growth in Atlantic salmon. It is our assumption that the nutrient value of this product, therefore, is different compared to the more traditional protein meal tested in Trial B. It may also influence the microbiota in the gut differently based on the same arguments. The results from trial C will be very important as this ensilage-based treatment has not yet been used at a routine level, so any new information on its effect on fish health and growth will be highly useful to the aquaculture industry at large.</p><p><br></p><p><strong>HoloFood Trial C - blue mussel ensilage-dose response</strong> is reported in the current study <a href='https://www.ebi.ac.uk/metabolights/MTBLS4384' rel='noopener noreferrer' target='_blank'><strong>MTBLS4384</strong></a>.</p><p><strong>HoloFood Trial A - seaweed-dose response</strong> is reported in <a href='https://www.ebi.ac.uk/metabolights/MTBLS4381' rel='noopener noreferrer' target='_blank'><strong>MTBLS4381</strong></a>.</p><p><strong>HoloFood Trial B - blue mussel-dose response</strong> is reported in <a href='https://www.ebi.ac.uk/metabolights/MTBLS4382' rel='noopener noreferrer' target='_blank'><strong>MTBLS4382</strong></a>.</p><p><strong>HoloFood Trial D - fermented seaweed open water-dose response</strong> is reported in <a href='https://www.ebi.ac.uk/metabolights/MTBLS6733' rel='noopener noreferrer' target='_blank'><strong>MTBLS6733</strong></a>.</p><p><strong>HoloFood Trial 1/2/3</strong> is reported in <a href='https://www.ebi.ac.uk/metabolights/MTBLS6988' rel='noopener noreferrer' target='_blank'><strong>MTBLS6988</strong></a>.</p><p><br></p><p><strong>Linked cross omic data sets:</strong></p><p>Nucleic acid data associated with this study are available in the European Nucleotide Archive (ENA): accession number <a href='https://www.ebi.ac.uk/ena/browser/view/PRJEB43192' rel='noopener noreferrer' target='_blank'>PRJEB43192</a>.</p><p>Metagenomic data associated with this study are available from MGnify under the Super Study '<a href='https://www.ebi.ac.uk/metagenomics/super-studies/holofood' rel='noopener noreferrer' target='_blank'>holofood</a>'.</p>