Project description:We investigate the effect of a functional feed for immunostimulation (peptidoglycan extract from bacterial cell wall with nucleotide formulation) on L. salmonis infection levels on Atlantic salmon Salmo salar, and on host and parasite gene expression profiles. Atlantic salmon smolts (~95 g) were fed a control diet, or a low or high dose immunostimulant diet, and then exposed to L. salmonis copepodids in three subsequent exposures. The transcriptome of salmon lice late in the infection attached to either the low dose diet or control diet hosts were compared using a 38K oligonucleotide microarray.
Project description:<p>An experimental trial was conducted on juvenile farmed Atlantic salmon (Salmo salar) in the framework of the HoloFood project, which aims to test the effect of novel sustainable feed additives in the feed of farmed salmon. Trial B blue mussel was used as a novel sustainable protein feed source in the diet of Atlantic salmon. Blue mussel represents an alternative, more sustainable protein source than traditional fishmeal. The blue mussel meal was produced by de-shelled blue mussels, which were subsequently spray-dried. It was integrated into the diet, mainly replacing fishmeal as the protein source.</p><p><br></p><p><strong>HoloFood Trial B - blue mussel-dose response</strong> is reported in the current study <a href='https://www.ebi.ac.uk/metabolights/MTBLS4382' rel='noopener noreferrer' target='_blank'><strong>MTBLS4382</strong></a>.</p><p><strong>HoloFood Trial A - seaweed-dose response</strong> is reported in <a href='https://www.ebi.ac.uk/metabolights/MTBLS4381' rel='noopener noreferrer' target='_blank'><strong>MTBLS4381</strong></a>.</p><p><strong>HoloFood Trial C - blue mussel ensilage-dose response</strong> is reported in <a href='https://www.ebi.ac.uk/metabolights/MTBLS4384' rel='noopener noreferrer' target='_blank'><strong>MTBLS4384</strong></a>.</p><p><strong>HoloFood Trial D - fermented seaweed open water-dose response</strong> is reported in <a href='https://www.ebi.ac.uk/metabolights/MTBLS6733' rel='noopener noreferrer' target='_blank'><strong>MTBLS6733</strong></a>.</p><p><strong>HoloFood Trial 1/2/3</strong> is reported in <a href='https://www.ebi.ac.uk/metabolights/MTBLS6988' rel='noopener noreferrer' target='_blank'><strong>MTBLS6988</strong></a>.</p><p><br></p><p><strong>Linked cross omic data sets:</strong></p><p>Nucleic acid data associated with this study are available in the European Nucleotide Archive (ENA): accession number <a href='https://www.ebi.ac.uk/ena/browser/view/PRJEB43192' rel='noopener noreferrer' target='_blank'>PRJEB43192</a>.</p><p>Metagenomic data associated with this study are available from MGnify under the Super Study '<a href='https://www.ebi.ac.uk/metagenomics/super-studies/holofood' rel='noopener noreferrer' target='_blank'>holofood</a>'.</p>
Project description:This study investigates host-specific gene expression of the Pacific salmon lice, Lepeophtheirus salmonis oncorhynchii, while parasitizing a resistant host (Coho salmon), two susceptible hosts (Atlantic salmon, Sockeye salmon), and a population with-held hosts (starved), over 48 hrs.
