Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Virulence OXA-48-Kp

ABSTRACT: Genomic virulence content of OXA-48-producing Klebsiella pneumoniae clinical isolates

PROVIDER: PRJEB55414 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR10776000_1.fastq.gz	Fastqsanger.gz
	ERR10776000_2.fastq.gz	Fastqsanger.gz
	ERR10776001_1.fastq.gz	Fastqsanger.gz
	ERR10776001_2.fastq.gz	Fastqsanger.gz
	ERR10776002_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 228

Similar Datasets

WGS of OXA-48-producing K. pneumoniae

Project description:Whole-genome analysis of endemic OXA-48-producing K. pneumoniae clinical isolates in Catalonia, Spain

| PRJEB27508 | ENA

OXA-48 dissemination during an outbreak

Project description:The molecular epidemiology of plasmid-based dissemination of OXA-48 during a hospital outbreak

| PRJEB34285 | ENA

Plasmid-based dissemination of OXA-48

Project description:The molecular epidemiology of plasmid-based dissemination of OXA-48 during a hospital outbreak

| PRJEB13016 | ENA

ECDC Klebsiella pneumoniae NDM-1 OXA-48

Project description:European study on Klebsiella pneumoniae carrying both NDM-1 and OXA-48

| PRJEB35890 | ENA

Transcriptional changes in response to OXA-23 overexpression in Acinetobacter baumannii ATCC 17978

Project description:Purpose: The goal of this study was to elucidate the collateral effects associated with OXA-23 overexpression on the Acinetobacter baumannii global transcriptome. Results: Besides the 99.73-fold increase in blaOXA-23 transcript upon IPTG induction, no other transcripts showed more than a 2-fold change compared to the wildtype control. This suggests that OXA-23 over expression to levels similarly observed in multi drug resistant A. baumannii clinical isolates does not effect the transcriptome.

2021-11-15 | GSE185203 | GEO

Clinical Evaluation of Advanced MALDI-TOF MS for Carbapenemase Subtyping in Gram-negative Isolates

Project description:The spread of carbapenemase-producing Enterobacterales (CPE) is emerging as a significant clinical concern in tertiary hospitals and in particular, long-term care facilities with deficiencies in infection control. This study aims to evaluate an advanced matrix-assisted laser desorption/ionization mass spectrometry (A-MALDI) method for the identification of carbapenemases and further discrimination of their subtypes in clinical isolates. The A-MALDI method was employed to detect CPE target proteins. Enhancements were made to improve detectability and mass accuracy through the optimization of MALDI-TOF settings and internal mass calibration. A total of 581 clinical isolates were analyzed, including 469 CPE isolates (388 KPC, 51 NDM, 40 OXA, and 2 GES) and 112 carbapenemase-negative isolates. Clinical evaluation of the A-MALDI demonstrated 100% accuracy and precision in identifying all the collected CPE isolates. Additionally, A-MALDI successfully discriminated individual carbapenemase subtypes (KPC-2 or KPC-3/4; OXA-48 or OXA-181 or OXA-232; GES-5 or GES-24) and also differentiated co-producing carbapenemase strains (KPC & NDM; KPC & OXA; KPC & GES; NDM & OXA), attributed to its high mass accuracy and simultaneous detection capability. A-MALDI is considered a valuable diagnostic tool for accurately identifying CPE and carbapenemase’s subtypes in clinical isolates. It may also aid in selecting appropriate antibiotics for each carbapenemase subtype. Ultimately, we expect that the A-MALDI method will contribute to preventing the spread of antibiotic resistance and improving human public health.

2024-11-25 | PXD046179 | Pride

Clinical Evaluation of Advanced MALDI-TOF MS for Carbapenemase Subtyping in Gram-negative Isolates

2024-11-26 | PXD058284 | Pride

Carbapenemase OXA-48 in Klebsiella pneumoniae from free-living marine mammals

Project description:Antimicrobial-resistant bacteria in free-living marine mammals detection of Klebsiella pneumoniae subspecies pneumoniae producing the carbapenemase OXA-48 indicates likely anthropogenic marine microbial pollution.

| PRJEB28252 | ENA

Pathogenicity of Clinical OXA-48 Isolates and Impact of the OXA-48 IncL Plasmid on Virulence and Bacterial Fitness.

Project description:OXA-48 is the most common carbapenemase in Enterobacterales in Germany and one of the most frequent carbapenemases worldwide. Several reports have associated bla OXA - 48 with a virulent host phenotype. To challenge this hypothesis, 35 OXA-48-producing clinical isolates of Escherichia coli (n = 15) and Klebsiella pneumoniae (n = 20) were studied in vitro, in vivo employing the Galleria mellonella infection model and by whole-genome sequencing. Clinical isolates belonged to 7 different sequence types (STs) in E. coli and 12 different STs in K. pneumoniae. In 26/35 isolates bla OXA- 48 was located on a 63 kb IncL plasmid. Horizontal gene transfer (HGT) to E. coli J53 was high in isolates with the 63 kb IncL plasmid (transconjugation frequency: ?103/donor) but low in isolates with non-IncL plasmids (<10-6/donor). Several clinical isolates were both highly cytotoxic against human cells and virulent in vivo. However, 63 kb IncL transconjugants generated from these highly virulent isolates were not more cytotoxic or virulent when compared to the recipient strain. Additionally, no genes associated with virulence were detected by in silico analysis of OXA-48 plasmids. The 63 kb plasmid was highly stable and did not impair growth or fitness in E. coli J53. In conclusion, OXA-48 clinical isolates in Germany are diverse but typically harbor the same 63 kb IncL plasmid which has been reported worldwide. We demonstrate that this 63 kb IncL plasmid has a low fitness burden, high plasmid stability and can be transferred by highly efficient HGT which is likely the cause of the rapid dissemination of OXA-48 rather than the expansion of a single clone or gain of virulence.

| S-EPMC6838017 | biostudies-literature

OXA-48 Klebsiella pneumoniae

Project description:Isolation of a carbapenemase producing Klebisiella in the NUH hospital

| PRJEB6416 | ENA