Project description:In this study, we have addressed the complex regulatory chloroplast quality-control pathway by modulating the expression of two nuclear genes encoding the plastid ribosomal proteins PRPS1 and PRPL4.
Project description:Biogenesis of plastid ribosomes is facilitated by auxiliary factors which process and modify ribosomal RNAs (rRNA) or are involved in ribosome assembly. In comparison to their bacterial and mitochondrial counterparts, the biogenesis of plastid ribosomes is less well understood and few auxiliary factors have been described so far. In this study, we report the functional characterization of CGL20 (CONSERVED ONLY IN THE GREEN LINEAGE20) in Arabidopsis thaliana (AtCGL20) a small, proline-rich protein which is targeted to mitochondria and chloroplasts. In Arabidopsis, CGL20 is encoded by segmentally duplicated genes of high similarity (AtCGL20A and AtCGL20B), and inactivation of both in the atcgl20ab mutant leads to a visible virescent phenotype, and growth arrest at low temperature. The chloroplast proteome, thylakoid complex abundance and pigment composition are significantly affected in atcgl20ab mutants, resulting in a combined proton gradient regulation (pgr) and chlororespiratory reduction (crr) phenotype. Loss of AtCGL20 alters plastid rRNA ratios, perturbs the formation of a hidden break in 23S rRNA and causes abnormal accumulation of 50S ribosomal subunits in the high-molecular-mass fraction of chloroplast stromal extracts. Moreover, AtCGL20A-eGFP fusion proteins comigrate with 50S ribosomal subunits in sucrose density gradients, even after RNase treatment of stromal extracts. Therefore, we propose that AtCGL20 participates in late stages of the biogenesis of 50S ribosomal subunits in plastids – a role which presumably evolved in the green lineage as a consequence of structural divergence of plastid ribosomes.
Project description:Signals originating within plastids modulate organelle differentiation by transcriptionally regulating nuclear-encoded genes. These retrograde signals are also integral regulators of plant development, including leaf morphology. The clb5 mutant displays severe leaf morphology defects due to Apocarotenoid Signal 1 (ACS1) accumulation in the developmentally arrested plastid. Transcriptomic analysis of clb5 validates ACS1 as a true retrograde signal impacting expression of hundreds of nuclear genes, including suppression of most genes encoding plastid ribosomal proteins.
2021-06-02 | GSE152252 | GEO
Project description:complete plastid genome of Lysimachia mauritiana
| PRJNA848685 | ENA
Project description:complete plastid genome of Hordeum agriocrithon
Project description:Series of 6 repetitions of hybridization of treatment (prpl11) and control (WT) each. Comparison of the Arabidopsis prpl11 mutant lacking the plastid ribosomal protein PRPL11 versus WT. P. Pesaresi et al., Plant J 27 (2001), pp. 179-189 Keywords: repeat sample
Project description:Series of 6 repetitions of hybridization of treatment (prpl11) and control (WT) each. Comparison of the Arabidopsis prpl11 mutant lacking the plastid ribosomal protein PRPL11 versus WT. P. Pesaresi et al., Plant J 27 (2001), pp. 179-189 Keywords: repeat sample