Ontology highlight
ABSTRACT:
PROVIDER: PRJEB58434 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Setup and optimisation of a high throughput pipeline for ChIPseq. The protocol used is ChIP carried out using the Agilent Bravo robot with subsequent Ilumina sequencing library preparation.This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
2013-02-12 | E-ERAD-77 | biostudies-arrayexpress
Project description:Laboratory tests with marine flatfish were conducted to investigate associations among gene expression, higher biological responses and wastewater effluent exposure. Previous studies showed molecular responses such as elevated concentrations of plasma estradiol and vitellogenin in wild male hornyhead turbot (Pleuronichthys verticalis). In the present study, male hornyhead turbot were exposed to environmentally realistic (0.5%) and higher (5%) concentrations of chemically enhanced advanced-primary (PL) and full-secondary treated (HTP) effluents from two southern California wastewater treatment plants (WWTP). Hepatic gene expression was examined using a custom low-density microarray. <br><br>
2013-12-10 | E-MEXP-3851 | biostudies-arrayexpress
Project description:We established a bacteria infective intestinal inflammation in turbot (Scophthalmus maximus). And found that β-glucan could significantly alleviate the phenotype of turbot intestinal inflammation. We performed single cell transcriptome analysis to study bacteria infective intestinal inflammation and the effects of β-glucan. Furthermore, we revealed that β-glucan through activates Th17 cells to alleviate intestinal inflammation in turbot.
2023-12-31 | GSE238068 | GEO
Project description:Cell types of turbot blood leukocytes remian unknown. We used single cell RNA sequencing (scRNA-seq) to analyze the cell types of turbot blood leukocytes.
2023-04-30 | GSE224979 | GEO
Project description:To investigate the impact of pathogenic immune stimulation on the lipid droplet (LD) proteome of turbot (Scophthalmus maximus), turbot were intraperitoneally injected with PBS (XA01292LQ_P), wild-type Edwardsiella piscicida EIB202 (XA01292LQ_W), or inactivated E. piscicida EIB202 (XA01292LQ_T). Liver tissues were collected, and LDs were extracted for 4D label-free quantitative proteomics analysis.
2024-11-21 | PXD047605 | Pride
Project description:Our present study analyzes decidual natural killer(dNK) cells expression profiles of the lncRNA and mRNA in missed abortional patients. A total of 276 mRNAs and 67 lncRNAs were identified to be significantly dysregulated in missed abortion (p<0.01). Subsequently, the potential function of dysregulated mRNAs and lncRNAs in missed abortion were explored through the bioinformatics method. In addition, the relationship between these dysregulated lncRNAs and mRNAs was revealed through constructing the lncRNA-mRNA interaction network. Our data showed that upregulated mRNAs were enriched in immune response and cytokine-cytokine receptor interaction while downregulated mRNAs were mainly related to cell adhesion and ECM-receptor interaction. Most importantly, several novel lncRNAs in dNKs, regulating core maternal-fetal interface activation-related mRNAs, were algorithmically predicted, indicating involvement of lncRNAs in the pathogenesis in early missed abortion.Topology analysis of lncRNA-mRNA interaction network suggested that the network presented a small world property, which means that most of mRNAs in the network were regulated by relative small number of hub lncRNAs. This study paves the way of investigating pathogenesis of early human missed abortion and facilitating the development of novel missed abortion therapeutics targeting lncRNAs.
2020-10-15 | GSE139087 | GEO
Project description:Scophthalmus maximus (turbot), genomic and transcriptomic data
| PRJEB69509 | ENA
Project description:ChIP sequencing of chordoma UCH-1 cell line. A well characterised chordoma cell line which bears all the morphological and immunohistochemical features of a chordoma (large vacuolated slow-growing cells, brachyury and cytokeratin-positive) have been used in this study. Chromatin immunoprecipitation using a well characterised anti-Brachyury antibody which has been used in immunohistochemistry of chordoma samples and previous ChIP studies in other systems. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
2011-10-17 | E-ERAD-23 | biostudies-arrayexpress