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Whole genome sequencing of adaptors selected by sub-inhibitory amount of brefeldin A in Candida albicans

ABSTRACT: Whole genome sequencing of adaptors selected by sub-inhibitory amount of brefeldin A in Candida albicans

PROVIDER: PRJEB58825 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR10760752_1.fastq.gz	Fastqsanger.gz
	ERR10760752_2.fastq.gz	Fastqsanger.gz
	ERR10760753_1.fastq.gz	Fastqsanger.gz
	ERR10760753_2.fastq.gz	Fastqsanger.gz
	ERR10760754_1.fastq.gz	Fastqsanger.gz

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Whole genome sequencing of adaptors selected by sub-inhibitory amount of brefeldin A in Candida albicans

Project description:In Candida albicans, the lab strain SC5314 was grown in YPD broth supplemented with 4ug/ml brefeldin A for 24h. The culture was washed, diluted and spread on YPD. Randomly 120 colonies were tested for tolerance to brefeldin A. Two adaptors (FY200 and FY201) obtained tolerance. These two adaptors were sequenced.

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Project description:Cryptococcus neoformans lab strain H99 was spread on YPD plates supplemented with 64ug/ml brefeldin A. Randomly 60 adaptors were chosen. These adaptors, as well as the parent were sequenced.

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Whole genome sequencing of BG2 derived adaptors selected from sub-inhibitory amount of tunicamycin

Project description:Candida albicans lab strain BG2 was grown in YPD broth supplemented with 0.5ug/ml tunicamycin at 37C for 24h. We used three techinical repeats. The culture was washed, and diluted with distilled water. Then approximately 200 cells from each of the three cultures were spread on YPD plates. Randomly 99 colonies from each culture were tetsted for tolerance to tunicamycin, as compared to the parent BG2. In total we got 6 tolerant adaptors. These 6 adaptors as well as the parent were sequenced.

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Project description:Cryptococcus neoformans lab strain H99 was grown in YPD broth supplemented with 16ug/ml brefeldin A for 72h at 30C. We used 3 biological repeats. Then the culture was washed and diluted with distilled water, then plated on YPD plate. From each culture, we randomly tested 120 colonies to see if they obtained tolerance to brefeldin A. In total, we got 30 tolerant adaptors. We sequenced the adaptors as well as the parent.

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Project description:Candida albicans lab strain SC5314 was exposued to sub-inhibitory amount (0.5 ug/ml) fluconazole for 24h. Two tolerant adaptors (FY1284 and FY1285) were obtained. The adaptors were unstable on YPD plate, yielding small (S) and large (L) colonies. The small (FY1284-S and FY1285-S) and the large (FY1284-L and FY1285-L) colonies were sequenced

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